Elongin A Antibody - BSA Free

Western Blot: Elongin A Antibody - BSA Free [NBP3-45280] - HeLa cells were subjected to SDS PAGE followed by western blot with (Elongin A antibody) at dilution of 1:1000

Immunohistochemistry: Elongin A Antibody - BSA Free [NBP3-45280] - Immunohistochemistry of paraffin-embedded human kidney cancer using (Elongin A antibody) at dilution of 1:100

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Elongin A Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Elongin A Antibody - BSA Free (NBP3-45280) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A). (Uniprot# Q14241)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TCEB3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 105 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Elongin A Antibody - BSA Free

• EloA
• Elongin 110 kDa subunit
• elongin-A
• FLJ38760
• FLJ42849
• RNA polymerase II transcription factor SIII subunit A1
• SIII p110
• SIII
• TCEB3A
• transcription elongation factor B (SIII), polypeptide 3 (110kD, elongin A)
• transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A)
• transcription elongation factor B alpha subunit
• transcription elongation factor B polypeptide 3

Background

TCEB3 (Elongin A) is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TCEB3