ELAC2 Antibody - BSA Free

Western Blot: ELAC2 Antibody [NBP2-97772] - Anti-ELAC2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 95 kDa

Immunoprecipitation: ELAC2 Antibody [NBP2-97772] - ELAC2 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-ELAC2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ELAC2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 95 kDa

Immunocytochemistry/Immunofluorescence: ELAC2 Antibody [NBP2-97772] - Immunofluorescence staining of ELAC2 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97772 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.

• Reactivity: Hu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ELAC2 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human ELAC2 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ELAC2
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunoprecipitation 0.5-2uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for ELAC2 Antibody - BSA Free

• EC 3.1.26.11
• elaC homolog 2 (E. coli)
• ElaC homolog protein 2
• ELC2
• FLJ10530
• FLJ36693
• FLJ42848
• Heredity prostate cancer protein 2
• HPC2elaC (E. coli) homolog 2
• putative prostate cancer susceptibility protein HPC2/ELAC2
• Ribonuclease Z 2
• RNase Z 2
• tRNA 3 endonuclease 2
• tRNase Z (long form)
• tRNase Z 2
• zinc phosphodiesterase ELAC protein 2

Background

The protein encoded by the ELAC2 gene has a C-terminal domain with tRNA 3′ processing endoribonuclease activity,which catalyzes the removal of the 3' trailer from precursor tRNAs. The protein also interacts with activatedSmad family member 2 (Smad2) and its nuclear partner forkhead box H1 (also known as FAST-1), and reduced expressioncan suppress transforming growth factor-beta induced growth arrest. Mutations in this gene result in an increased riskof prostate cancer. Multiple transcript variants encoding different isoforms have been found for this gene. (providedby RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ELAC2