RBBP6 Antibody

Images

 
Western Blot: RBBP6 Antibody [NBP1-49535] - Analysis of RbBP6 in HeLa nuclear extracts.
Immunohistochemistry-Paraffin: RBBP6 Antibody [NBP1-49535] - IHC analysis of formalin-fixed paraffin-embedded tissue section from intestine of normal mouse with 1:400 dilution of RBBP6 antibody. The intestinal ...read more
Immunohistochemistry-Paraffin: RBBP6 Antibody [NBP1-49535] - IHC analysis of formalin-fixed paraffin-embedded tissue section from skin of normal mouse with 1:400 dilution of RBBP6 antibody. The keratinocytes and other ...read more
Immunoprecipitation: RBBP6 Antibody [NBP1-49535] - Immunoprecipitation of RbBP6 from HeLa cells using A. NBP1-49542 and B. NBP1-49535.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
 

Independent Antibodies

       

RBBP6 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human RBBP6 protein (between residues 1600-1650) [UniProt Q7Z6E9]
Localization
Nucleus nucleolus. Chromosome. Note: Colocalizes with mitotic chromosomes.
Specificity
This antibody may detect isoform 1 (201.6 kDa), isoform 2 (197.3 kDa) and isoform 4 (106.0 kDa) of human RBBP6.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RBBP6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:400
  • Immunoprecipitation 1:10-1:500
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27667193).
Positive Control
HeLa Lysate (NB800-PC1)
Publications
Read Publications using
NBP1-49535 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RBBP6 Antibody

  • E3 ubiquitin-protein ligase RBBP6
  • EC 6.3.2.-
  • MY038
  • P2PR
  • P2P-R
  • p53-associated cellular protein of testis
  • PACTDKFZp761B2423
  • Proliferation potential-related protein
  • Protein P2P-R
  • RB-binding Q-protein 1
  • RBQ1
  • RBQ-1
  • retinoblastoma binding protein 6
  • Retinoblastoma-binding protein 6DKFZp686P0638
  • Retinoblastoma-binding Q protein 1
  • SNAMA

Background

RBBP6 (retinoblastoma binding protein 6; P2PR, PACT, RBQ1) is a multidomain protein with an expected molecular size of about 250kD and it colocalizes with mitotic chromosomes as well as with NEK6 in centrosomes. RBBP6 is highly expressed in normal placenta and testis, and is overexpressed in certain cancers (esophageal and lung cancer). RBBP6 is a multifunctional protein which is found presumably in all eukaryotes and is implicated in versatile cellular activities such as mRNA metabolism, cell cycle regulation, carcinogenesis, and development. It forms part of the pre-mRNA 3-end processing complex in humans and the cleavage/polyadenylation factor complex in yeast. RBBP6 interacts with p53/TP53, RB1, NEK6, MDM2, YBX1 etc., and gets phosphorylated upon DNA damage via ATM/ATR or through NEK6. RBBP6 is an E3 ubiquitin-protein ligase which promotes ubiquitination of YBX1, thereby facilitating its proteasomal degradation. RBBP6 also acts as a scaffold protein to enhance the assembly of the p53/TP53-MDM2 complex, resulting in increase of MDM2-mediated ubiquitination and degradation of p53/TP53, thereby acting as a negative regulator of p53/TP53.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-49535
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Applications: WB, ICC/IF, IHC, IHC-P, IP

Publications for RBBP6 Antibody (NBP1-49535)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(1)
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(1)
Mouse
(1)
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Product General Protocols

View specific protocols for RBBP6 Antibody (NBP1-49535): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RBBP6 Antibody (NBP1-49535) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-49535AF405
Alexa Fluor 488 Labeled NBP1-49535AF488
Alexa Fluor 647 Labeled NBP1-49535AF647
Alexa Fluor 700 Labeled NBP1-49535AF700
Biotin Labeled NBP1-49535B
DyLight 405 Labeled NBP1-49535V
DyLight 488 Labeled NBP1-49535G
DyLight 550 Labeled NBP1-49535R
DyLight 650 Labeled NBP1-49535C
FITC Labeled NBP1-49535F
HRP Labeled NBP1-49535H

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Bioinformatics

Gene Symbol RBBP6
Entrez
Uniprot