eIF4GII Antibody (1H5) - Azide and BSA Free

Western Blot: eIF4GII Antibody (1H5) [H00008672-M03] - Analysis of EIF4G3 expression in MCF-7 (Cat # L046V1).

Immunohistochemistry-Paraffin: eIF4GII Antibody (1H5) [H00008672-M03] - Analysis of monoclonal antibody to EIF4G3 on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. Antibody concentration 3 ug/ml

Western Blot: eIF4GII Antibody (1H5) [H00008672-M03] - EIF4G3 over-expressed 293 cell line, cotransfected with EIF4G3 Validated Chimera RNAi or non-transfected control. Blot probed with H00008672-M03. GAPDH (36.1 kDa) used as loading control.

Western Blot: eIF4GII Antibody (1H5) [H00008672-M03] - Analysis of EIF4G3 expression in HeLa (Cat # L013V1).

Western Blot: eIF4GII Antibody (1H5) [H00008672-M03] - Analysis of EIF4G3 expression in transfected 293T cell line by EIF4G3 monoclonal antibody (M03), clone 1H5. Lane 1: EIF4G3 transfected lysatE (55.2 KDa). Lane 2: Non-transfected lysate.

ELISA: eIF4GII Antibody (1H5) [H00008672-M03] - Detection limit for recombinant GST tagged EIF4G3 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, KD
• Clone: 1H5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

eIF4GII Antibody (1H5) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: EIF4G3 (AAH30578, 1 a.a. ~ 515 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNSQPQTRSPFAAGPRPPHHQFFQRPQIQPPRATIPNSSPSIRPGAQTPTAVYQANQHIMMVNHLPMPYPVPQGPQYCIPQYRHSGPPYVGPPQQYPVQPPGPGPFYPGPGPGDFPNAYGTPFYPSQPVYQSAPIMVPTQQQPPPAKREKKTIRIRDPNQGGKDITEEIISGGGSRNPTPPIGRPTSTPTPPQQLPSQVPEHSPVVYGTVESAHLAASTPVTAASDQKQEEKPKPDPVLKSPSPVLRLVLSGEKKEQEGQTSETTAIVSIAELPLPPSPTTVSSVARSTIAAPTSSALSSQPIFTTAIDDRCELSSPREDTIPIPSLTSCTETSDPLPTNENDGDICKKPCSVAPNDIPLVSSTNLINEINGVSEKLSATESIVEIVKQEVLPLTLELEILENPPEEMKLECIPAPITPSTVPSFPPTPPTPPASPPHTPVIVPAAATTVSSPSAAITVQRVLEEDESIRTCLSEDAKEIQNKIEVEADGQTEEILDSQNLNSRRSPVPETSNEC
• Specificity: EIF4G3 - eukaryotic translation initiation factor 4 gamma, 3
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EIF4G3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for eIF4GII Antibody (1H5) - Azide and BSA Free

• eIF-4G 3
• eIF-4-gamma 3
• eIF-4-gamma II
• eIF4GIIeIF4G 3
• eukaryotic translation initiation factor 4 gamma 3
• eukaryotic translation initiation factor 4 gamma, 3

Background

Probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF4G3
• Entrez:
  • 8672(Human)
• OMIM:
  • 603929(Human)
• Uniprot:
  • AAH30578(Human)