eIF4G1 Antibody

Product Discontinued

eIF4G1 Antibody Summary

• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human eIF4G around the phosphorylation site of serine 1232 (P-V-Sp-P-L).
• Specificity: eIF4G (Ab-1232) detects endogenous levels of total eIF4G protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF4G1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 220 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl and 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for eIF4G1 Antibody

• DKFZp686A1451
• EIF4F
• eIF-4G 1
• eIF4G1
• EIF-4G1
• eIF-4-gamma 1
• EIF4-gamma
• EIF4GI
• EIF4Gp220
• eucaryotic translation initiation factor 4G
• eukaryotic translation initiation factor 4 gamma 1
• eukaryotic translation initiation factor 4 gamma, 1
• P220

Background

eIF4G1 (eukaryotic translation Initiation Factor 4 Gamma 1) is a component of the protein complex eIF-4 which is involved in the recognition of the mRNA cap ATP-dependent unwinding of the 5'-terminal secondary structure and recruitment of mRNA to the ribosome. eIF4G plays a critical role in protein expression and is at the center of a complex regulatory network. Together with the cap-binding protein eIF4E, it recruits the small ribosomal subunit to the 5'-end of mRNA and promotes the assembly of a functional translation initiation complex which scans along the mRNA to the translation start codon. Human eIF4G contains three consecutive HEAT domains, as well as long unstructured regions involved in multiple protein-protein interactions. The interactions of eIF4G1 with other factors are largely unknown.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for eIF4G1 Antibody (NBP1-04964)(1)

Publication using NBP1-04964

• Dell'Anno I, Barbarino M, Barone E et al. EIF4G1 and RAN as Possible Drivers for Malignant Pleural Mesothelioma Int J Mol Sci 2020-07-09 [PMID: 32659970]

Bioinformatics

• Gene Symbol: EIF4G1
• Entrez:
  • 1981(Human)
• Uniprot:
  • Q04637()