eIF4E [p Ser209] Antibody (SU0396)

Western Blot: eIF4E [p Ser209] Antibody (SU0396) [NBP2-66802] - Analysis of eIF4E [p Ser209] on NIH-3T3 cell lysates. Lane 1: NIH-3T3 cells, whole cell lysate, 10 ug /lane. Lane 2: NIH-3T3 cells were treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-eIF4E [p Ser209] (1/500) and Anti-GAPDH antibody (1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 2 minutes 29 seconds

Immunocytochemistry/Immunofluorescence: eIF4E [p Ser209] Antibody (SU0396) [NBP2-66802] - Staining Phospho-eIF4E(S209) in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: eIF4E [p Ser209] Antibody (SU0396) [NBP2-66802] - Analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-Phospho-eIF4E (S209) antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: eIF4E [p Ser209] Antibody (SU0396) [NBP2-66802] - Analysis of Phospho-eIF4E(S209) on different lysates using anti-Phospho-eIF4E(S209) antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse spleen Lane 2: Rat spleen

Immunocytochemistry/Immunofluorescence: eIF4E [p Ser209] Antibody (SU0396) [NBP2-66802] - Staining Phospho-eIF4E(S209) in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: eIF4E [p Ser209] Antibody (SU0396) [NBP2-66802] - Staining Phospho-eIF4E(S209) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: eIF4E [p Ser209] Antibody (SU0396) [NBP2-66802] - Analysis of paraffin-embedded human spleen tissue using anti-Phospho-eIF4E(S209) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: eIF4E [p Ser209] Antibody (SU0396) [NBP2-66802] - Analysis of paraffin-embedded mouse spleen tissue using anti-Phospho-eIF4E(S209) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: eIF4E [p Ser209] Antibody (SU0396) [NBP2-66802] - Analysis of paraffin-embedded rat spleen tissue using anti-Phospho-eIF4E(S209) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: eIF4E [p Ser209] Antibody (SU0396) [NBP2-66802] - Analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Phospho-eIF4E (S209) antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: eIF4E [p Ser209] Antibody (SU0396) [NBP2-66802] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-eIF4E (S209) antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

eIF4E [p Ser209] Antibody (SU0396) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser209 of human eIF4E. (SwissProt: P06730 Human; SwissProt: P63073 Mouse; SwissProt: P63074 Rat)
• Modification: p Ser209
• Localization: Cytoplasm, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: EIF4E
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for eIF4E [p Ser209] Antibody (SU0396)

• CBP
• eIF4E
• eIF-4E
• EIF4E1
• EIF4EL1
• EIF4EL1MGC111573
• eIF-4F 25 kDa subunit
• EIF4F
• eukaryotic translation initiation factor 4E
• eukaryotic translation initiation factor 4E-like 1
• mRNA cap-binding protein

Background

eIF-4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It exists in two forms: as a free form (25 kDa) and as part of a multiprotein complex eIF-4F (1). eIF-4E appears to be the least abundant of the initiation factors and acts as a rate-limiting step of initiation (2). Since translation is regulated by phosphorylation, eIF-4E phosphorylation at Ser 209 by MAPK signal-integrating kinase 1 (Mnk1) and kinase 2 (Mnk2) may directly regulate the rate of protein synthesis initiation (3). There is also evidence that eIF-4E can function as an oncogene (4-5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for eIF4E Antibody (NBP2-66802)(1)

Publication using NBP2-66802

• Bou-Petit E, HUmmer S, Alarcon H et al. Overcoming Paradoxical Kinase Priming by a Novel MNK1 Inhibitor Journal of medicinal chemistry 2022-04-28 [PMID: 35417652] (WB, Human)

Bioinformatics

• Gene Symbol: EIF4E