eIF3K Antibody - BSA Free

Western Blot: eIF3K Antibody [NBP1-31285] - Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE; antibody diluted at 1:1000.

eIF3K antibody [N1C2] detects eIF3K protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: eIF3K protein stained by eIF3K antibody [N1C2] (NBP1-31285) diluted at 1:500.Blue: Hoechst 33343 staining.

• Reactivity: Hu, Mu, Bv, RM, Xp
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

eIF3K Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit eIF3K Antibody - BSA Free (NBP1-31285) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human eIF3K. The exact sequence is proprietary.
• Localization: Nucleus, Cytoplasm
• Predicted Species: Mouse (100%), Rhesus Macaque (100%), Bovine (100%), Xenopus (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF3K
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Protein A purified

Alternate Names for eIF3K Antibody - BSA Free

• ARG134
• eIF3k
• EIF3-p28
• EIF3S12eIF-3 p28
• Eukaryotic translation initiation factor 3 subunit 12
• eukaryotic translation initiation factor 3 subunit K
• eukaryotic translation initiation factor 3, subunit 12
• eukaryotic translation initiation factor 3, subunit K
• HSPC029
• M9
• MSTP001
• muscle specific
• Muscle-specific gene M9 protein
• PLAC24
• PLAC-24
• PLAC-24eIF-3 p25
• PRO1474
• PTD001

Background

The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: EIF3K
• Entrez:
  • 515326(Bovine)
  • 27335(Human)
  • 73830(Mouse)
• Uniprot:
  • Q9UBQ5(Human)