eIF3K Antibody

Images

 
Western Blot: eIF3K Antibody [NBP1-31285] - Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: eIF3K Antibody [NBP1-31285] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF3K protein stained by EIF3K antibody diluted at 1:500. Blue: ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, RM, XpSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
10 mg/ml

Order Details

eIF3K Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human EIF3K. The exact sequence is proprietary.
Localization
Nucleus, Cytoplasm
Predicted Species
Mouse (100%), Rhesus Macaque (100%), Bovine (100%), Xenopus (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF3K
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (88%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
10 mg/ml
Purity
Protein A purified

Alternate Names for eIF3K Antibody

  • ARG134
  • eIF3k
  • EIF3-p28
  • EIF3S12eIF-3 p28
  • Eukaryotic translation initiation factor 3 subunit 12
  • eukaryotic translation initiation factor 3 subunit K
  • eukaryotic translation initiation factor 3, subunit 12
  • eukaryotic translation initiation factor 3, subunit K
  • HSPC029
  • M9
  • MSTP001
  • muscle specific
  • Muscle-specific gene M9 protein
  • PLAC24
  • PLAC-24
  • PLAC-24eIF-3 p25
  • PRO1474
  • PTD001

Background

The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EIF3K
Entrez
Uniprot