eIF3e Antibody

Images

 
Western Blot: eIF3e Antibody [NBP2-16298] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji 10% SDS PAGE gel, diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: eIF3e Antibody [NBP2-16298] - HeLa cells were fixed in iced-cold MeOH for 5 min. Green: EIF3E protein stained by eIF3e antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Immunohistochemistry-Paraffin: eIF3e Antibody [NBP2-16298] - Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.
Western Blot: eIF3e Antibody [NBP2-16298] - Sample (30 ug of whole cell lysate) A: BCL-1 10% SDS PAGE gel, diluted at 1:2000.
Immunohistochemistry-Paraffin: eIF3e Antibody [NBP2-16298] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

eIF3e Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human eIF3e. The exact sequence is proprietary.
Localization
Cytoplasm; Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF3E
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for eIF3e Antibody

  • eIF-3 p48
  • eIF3e
  • eIF3-p48
  • EIF3S6EIF3-P48
  • Eukaryotic translation initiation factor 3 subunit 6
  • eukaryotic translation initiation factor 3 subunit E
  • eukaryotic translation initiation factor 3, subunit 6 (48kD)
  • eukaryotic translation initiation factor 3, subunit 6 48kDa
  • eukaryotic translation initiation factor 3, subunit E
  • INT6eIF3-p46
  • mammary tumor-associated protein INT6
  • murine mammary tumor integration site 6 (oncogene homolog)
  • Viral integration site protein INT-6 homolog

Background

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol EIF3E
Uniprot