eIF3e Antibody

Western Blot: eIF3e Antibody [NBP2-16298] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji 10% SDS PAGE gel, diluted at 1:2000.

Immunocytochemistry/Immunofluorescence: eIF3e Antibody [NBP2-16298] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: EIF3E protein stained by eIF3e antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: eIF3e Antibody [NBP2-16298] - Cal27 xenograft, using antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

Western Blot: eIF3e Antibody [NBP2-16298] - Sample (30 ug of whole cell lysate) A: BCL-1 10% SDS PAGE gel, diluted at 1:2000.

Immunohistochemistry-Paraffin: eIF3e Antibody [NBP2-16298] - C2C12 xenograft, using eIF3e antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

• Reactivity: Hu, Mu, Bv, Ch, RM, Xp
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

eIF3e Antibody Summary

• Description: Novus Biologicals Rabbit eIF3e Antibody (NBP2-16298) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human eIF3e. The exact sequence is proprietary.
• Localization: Cytoplasm; Nucleus
• Predicted Species: Bovine (100%), Rhesus Macaque (100%), Xenopus (97%), Chicken (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF3E
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for eIF3e Antibody

• eIF-3 p48
• eIF3e
• eIF3-p48
• EIF3S6EIF3-P48
• Eukaryotic translation initiation factor 3 subunit 6
• eukaryotic translation initiation factor 3 subunit E
• eukaryotic translation initiation factor 3, subunit 6 (48kD)
• eukaryotic translation initiation factor 3, subunit 6 48kDa
• eukaryotic translation initiation factor 3, subunit E
• INT6eIF3-p46
• mammary tumor-associated protein INT6
• murine mammary tumor integration site 6 (oncogene homolog)
• Viral integration site protein INT-6 homolog

Background

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: EIF3E
• Uniprot:
  • P60228(Human)