EIF2 beta Antibody

Western Blot: EIF2 beta Antibody [NB100-93283] - Detection of Human and Mouse eIF2beta/EIF2S2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit antieIF2beta/ EIF2S2 antibody NB100-93283 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. eIF2beta/EIF2S2 was immunoprecipitated more efficiently by rabbit anti-eIF2beta/EIF2S2 antibody NB100-93284, which recognizes a downstream epitope.

• Reactivity: Hu, Mu, Pm
• Applications: WB, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

EIF2 beta Antibody Summary

• Description: Novus Biologicals Rabbit EIF2 beta Antibody (NB100-93283) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 75 and 125 of human eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa using the numbering given in entry NP_003899.2 (GeneID 8894).
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF2S2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000-1:10000
• Application Notes: Not recommended for immunoprecipitation.
• Control:

  EIF2 beta Overexpression Lysate

Reactivity Notes

Orangutan (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EIF2 beta Antibody

• DKFZp686L18198
• EIF2
• EIF2B
• EIF2beta
• eIF-2-beta
• eukaryotic initiation factor 2-beta
• eukaryotic translation initiation factor 2 beta
• eukaryotic translation initiation factor 2 subunit 2
• Eukaryotic translation initiation factor 2 subunit beta
• eukaryotic translation initiation factor 2, subunit 2 (beta, 38kD )
• eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa
• MGC8508

Background

Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF2S2
• Entrez:
  • 8894(Human)
  • 67204(Mouse)
• Uniprot:
  • NP_003899.2(Human)