EHD2 Antibody

Immunohistochemistry-Paraffin: EHD2 Antibody [NBP3-12299] - A) Mouse lung, B) Rat spleen. 1:500 antibody dilution in DiluObuffer. Apparent MW is 65 KDa.

Immunohistochemistry-Paraffin: EHD2 Antibody [NBP3-12299] - Mouse lung. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.

Immunohistochemistry-Paraffin: EHD2 Antibody [NBP3-12299] - Mouse lung. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

EHD2 Antibody Summary

• Description: Novus Biologicals Rabbit EHD2 Antibody (NBP3-12299) is a polyclonal antibody validated for use in IHC, WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide taken within amino acid 420-450 on human EH-Domain Containing Protein 2.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EHD2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation 1:200
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for EHD2 Antibody

• EH domain containing 2
• EH domain-containing protein 2
• EH-domain containing 2
• FLJ96617
• PAST homolog 2
• PAST2

Background

EHD2 encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EHD2