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EHD2 Antibody

Images

 
Western Blot: Goat Polyclonal EHD2 Antibody [NB100-1458] - (0.1g/ml) staining of Human Lung (A), Colon (B) and Placenta (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: EHD2 Antibody [NB100-1458] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by ...read more
Immunocytochemistry/ Immunofluorescence: EHD2 Antibody [NB100-1458] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by ...read more
Flow Cytometry: EHD2 Antibody [NB100-1458] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

EHD2 Antibody Summary

Immunogen
Peptide with sequence CRLVPPSKRRHKGSA corresponding to C-Terminus according to NP_055416.2.
Localization
Nuclear
Specificity
This antibody is expected to recognise EHD1 protein as well as EHD2.
Predicted Species
Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EHD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 - 0.3 ug/mL
Application Notes
Approx 70-75kDa band observed in Human Lung , Colon and Placenta lysates (calculated MW of 61.2kDa according to NP_055416.2). The observed molecular weight is observed by other sources. Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature.
Control
EHD2 Overexpression Lysate
Human Heart Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publication using NB100-1458.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EHD2 Antibody

  • EH domain containing 2
  • EH domain-containing protein 2
  • EH-domain containing 2
  • FLJ96617
  • PAST homolog 2
  • PAST2

Background

EHD2 is homologous to human EHD1. The deduced protein identities between EHD1 and EHD2, between EHD1 and EHD3, and between EHD1 and EHD4, are 71%, 86%, and 76%, respectively. All contain multiple conserved regions that include a nucleotide binding consensus site at the N terminus, a bipartite nuclear localization signal, and an eps15 homology (EH) protein binding domain with an EF hand motif at the C terminus. Northern blot analysis detected a EHD2 transcript that is highly expressed in heart and moderately expressed in placenta, lung, and skeletal muscle, as well as different transcript that is expressed at low levels in heart and skeletal muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EHD2 Antibody (NB100-1458)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol EHD2
Entrez
Uniprot