EHD2 Antibody

Western Blot: EHD2 Antibody [NB100-1458] - Staining of Human Lung lysate with antibody at 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: EHD2 Antibody [NB100-1458] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Flow Cytometry: EHD2 Antibody [NB100-1458] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Western Blot: EHD2 Antibody [NB100-1458] - Staining of A549 (A) with antibody at 0.1 ug/mL and HeLa (B) cell lysate with antibody at 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: EHD2 Antibody [NB100-1458] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, Flow, ICC/IF, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

EHD2 Antibody Summary

• Immunogen: Peptide with sequence CRLVPPSKRRHKGSA corresponding to C-Terminus according to NP_055416.2.
• Localization: Nuclear
• Specificity: This antibody is expected to recognise EHD1 protein as well as EHD2.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EHD2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 - 0.3 ug/mL
• Application Notes: Western blot: Approx 65-70kDa band observed in Human Lung lysates and in lysates of cell lines A549 and HeLa (calculated MW of 61.2kDa according to NP_055416.2). This molecular weight is routinely observed by other sources.
• Control:

  EHD2 Overexpression Lysate
  Human Heart Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EHD2 Antibody

• EH domain containing 2
• EH domain-containing protein 2
• EH-domain containing 2
• FLJ96617
• PAST homolog 2
• PAST2

Background

EHD2 is homologous to human EHD1. The deduced protein identities between EHD1 and EHD2, between EHD1 and EHD3, and between EHD1 and EHD4, are 71%, 86%, and 76%, respectively. All contain multiple conserved regions that include a nucleotide binding consensus site at the N terminus, a bipartite nuclear localization signal, and an eps15 homology (EH) protein binding domain with an EF hand motif at the C terminus. Northern blot analysis detected a EHD2 transcript that is highly expressed in heart and moderately expressed in placenta, lung, and skeletal muscle, as well as different transcript that is expressed at low levels in heart and skeletal muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EHD2 Antibody (NB100-1458)(1)

Publication using NB100-1458

• Guilherme A, Soriano NA, Furcinitti PS, Czech MP. Role of EHD1 and EHBP1 in perinuclear sorting and insulin-regulated GLUT4 recycling in 3T3-L1 adipocytes. J Biol Chem 2004-09-17 [PMID: 15247266]

Bioinformatics

• Gene Symbol: EHD2
• Entrez:
  • 30846(Human)
• Uniprot:
  • NP_055416.(Human)