EHD2 Antibody

Images

 
Western Blot: EHD2 Antibody [NB100-1458] - Staining of Human Placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: EHD2 Antibody [NB100-1458] - Staining of paraffin embedded Human Vessel. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

EHD2 Antibody Summary

Immunogen
Peptide with sequence CRLVPPSKRRHKGSA corresponding to C-Terminus according to NP_055416.2.
Localization
Nuclear
Specificity
This antibody is expected to recognise EHD1 protein as well as EHD2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EHD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 65 kDa band observed in human placenta lysates (calculated MW of 61.2 kDa band according to NP_055416.2). In transfected HEK293 transiently expressing EHD2 a band of approx. 62 kDa is observed. This band is not observed in the non-transfected HEK293. IHC: Paraffin embedded Human Vessel.
Positive Control
EHD2 Lysate (NBL1-10158)
Heart Lysate (NB820-59217)
Publications
Read Publication using NB100-1458.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EHD2 Antibody

  • EH domain containing 2
  • EH domain-containing protein 2
  • EH-domain containing 2
  • FLJ96617
  • PAST homolog 2
  • PAST2

Background

EHD2 is homologous to human EHD1. The deduced protein identities between EHD1 and EHD2, between EHD1 and EHD3, and between EHD1 and EHD4, are 71%, 86%, and 76%, respectively. All contain multiple conserved regions that include a nucleotide binding consensus site at the N terminus, a bipartite nuclear localization signal, and an eps15 homology (EH) protein binding domain with an EF hand motif at the C terminus. Northern blot analysis detected a EHD2 transcript that is highly expressed in heart and moderately expressed in placenta, lung, and skeletal muscle, as well as different transcript that is expressed at low levels in heart and skeletal muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EHD2 Antibody (NB100-1458)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EHD2
Entrez
Uniprot