EGLN2/PHD1 Antibody [Alexa Fluor™ Plus 488]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 488

EGLN2/PHD1 Antibody [Alexa Fluor™ Plus 488] Summary

• Immunogen: Partial synthetic peptide made to an N-terminal portion of the human EGLN2 / PHD1 protein (between amino acids 10-75) [UniProt Q96KS0]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EGLN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for EGLN2/PHD1 Antibody [Alexa Fluor™ Plus 488]

• DKFZp434E026
• EC 1.14.11
• EC 1.14.11.-
• egl nine homolog 2 (C. elegans)
• egl nine homolog 2
• EGLN2
• EIT6
• Estrogen-induced tag 6
• FLJ95603
• HIFPH1 EGL nine (C.elegans) homolog 2
• HIFPH1
• HIF-PH1
• HIF-prolyl hydroxylase 1
• HPH-1
• HPH-3
• Hypoxia-inducible factor prolyl hydroxylase 1
• PHD1
• Prolyl hydroxylase domain-containing protein 1

Background

HIF prolyl hydroxylase 1 (HPH-3 or EGLN2) is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EGLN2