EGLN2/PHD1 Antibody [Alexa Fluor® 750]

• Applications: WB, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 750

EGLN2/PHD1 Antibody [Alexa Fluor® 750] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human EGLN2/PHD1
  Asp2-Thr407
  Accession # Q96KS0
• Specificity: Detects human EGLN2/PHD1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

EGLN2 (EGL/EGg Laying-Nine #2; also known as PHD1 and HPH3) is a 43 kDa member of the EglN family of proteins. It is ubiquitously expressed and found principally in the nucleus. EGLN2 hydroxylates proline on HIF-1 alpha . HIF-1 is an alpha / beta heterodimeric transcriptional activator that upregulates genes involved in mitigating the effects of hypoxia. Normally, and in the presence of abundant oxygen, the HIF-1 alpha -chain is hydroxylated by PHD family members, which results in its ubiquitylation and degradation. Under low oxygen tension, EGLN2 activity is decreased, the HIF-1 alpha subunit is retained, and HIF-1 activates genes. Human EGLN2 is 407 amino acids (aa) in length (SwissProt #:Q96KS0). It contains one iron 2-oxoglutarate (Fe2OG) dioxygenase domain (aa 278-376) plus an iron-binding (His297 and His358), and a 2-oxoglutarate-binding (Arg367) site. There is one alternative start site at Met34 that generates a 40 kDa isoform. In addition, there is another potential splice form that shows a 16 aa substitution for aa 1-281. Full-length human EGLN2 shares 91% aa sequence identity with mouse EGLN2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.