EGFR Antibody (GFR450)

Immunohistochemistry-Paraffin: EGFR Antibody (GFR450) [NBP2-29440] - Formalin-fixed, paraffin-embedded squamous cell carcinoma stained with EGFR Ab (GFR450).

Flow Cytometry: EGFR Antibody (GFR450) [NBP2-29440] - Flow Cytometry of human EGFR on A431 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled EGFR Antibody (GFR450).

Immunofluorescence: EGFR Antibody (GFR450) [NBP2-29440] - Confocal Immunofluorescent analysis of A431 cells using AF488-labeled EGFR Monoclonal Antibody (GFR450) (Green). DAPI was used to stain the cell nuclei (blue).

Immunofluorescence: EGFR Antibody (GFR450) [NBP2-29440] - Confocal Immunofluorescent analysis of A431 cells using AF488-labeled Isotype Control Monoclonal Antibody (IgG2a) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control).

SDS-Page: EGFR Antibody (GFR450) [NBP2-29440]

Protein Array: EGFR Antibody (GFR450) [NBP2-29440] - Analysis of Protein Array containing more than 19,000 full-length human proteins using EGFR Antibody (GFR450). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

• Reactivity: Hu, Mu(-)
• Applications: Flow, IHC, IHC-P, PA, PAGE, ICC/IF
• Clone: GFR450
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

EGFR Antibody (GFR450) Summary

• Immunogen: Recombinant extracellular domain of human EGFR protein
• Localization: Cell surface
• Specificity: This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EGFR
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5-1ug/million cells in 0.1ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • Protein Array
  • SDS-Page
  • Immunofluorescence 1-2ug/ml
• Application Notes: Human Chromosome Location: 7p11.2
  Mol. Weight of Antigen: ~170kDa (wild type) and ~145kDa (vIII variant)
  Immunohistology (Formalin-fixed) (2-4ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein G purified

Alternate Names for EGFR Antibody (GFR450)

• avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
• cell growth inhibiting protein 40
• cell proliferation-inducing protein 61
• EC 2.7.10
• EC 2.7.10.1
• EGF R
• EGFR
• epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog)
• epidermal growth factor receptor
• ErbB
• ErbB1
• ERBB1PIG61
• HER1
• HER-1
• mENA
• Proto-oncogene c-ErbB-1
• Receptor tyrosine-protein kinase erbB-1

Background

The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER-1), Neu (ErbB-2, HER-2), ErbB-3 (HER-3) and ErbB-4 (HER-4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for EGFR Antibody (NBP2-29440). (Showing 1 - 1 of 1 FAQ).

1. I am looking at NBP2-29440-20ug. I see in your dilutions you say for IHC-P to us 0.5 ug/mL but for application notes it says 2-4 ug/mL --which is the recommended dilution?
   • If formalin fixed then you will want to try: Immunohistology (Formalin-fixed) (2-4ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). If not formalin fixed then Immunohistochemistry-Paraffin 0.5-1.0ug/ml. The dilutions are also just guidelines and we recommend optimizing for your own system.

Bioinformatics

• Gene Symbol: EGFR
• Uniprot:
  • P00533()