EGFR Antibody (423103) [Fluorescein]

A431 human epithelial carcinoma cell line was stained with Rat Anti-Human EGF R/ErbB1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB10951F, filled histogram) or isotype control antibody (Catalog # IC006F, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 423103
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Fluorescein

EGFR Antibody (423103) [Fluorescein] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human EGF R/ErbB1
  Leu25-Ser645
  Accession # CAA25240
• Specificity: Detects human EGF R/ErbB1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ErbB2, rhErbB3, or rhErbB4 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: EGFR
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EGFR Antibody (423103) [Fluorescein]

• avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
• cell growth inhibiting protein 40
• cell proliferation-inducing protein 61
• EC 2.7.10
• EC 2.7.10.1
• EGF R
• EGFR
• epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog)
• epidermal growth factor receptor
• ErbB
• ErbB1
• ERBB1PIG61
• HER1
• HER-1
• mENA
• Proto-oncogene c-ErbB-1
• Receptor tyrosine-protein kinase erbB-1

Background

Epidermal Growth Factor Receptor (EGF R), also named erythroblastic leukemia viral oncogene homolog 1 (ErbB1), is a member of the type I receptor tyrosine kinase superfamily. The epidermal growth factor receptor (EGF R) subfamily of receptor tyrosine kinases comprises four members: EGF R (also known as HER1, ErbB1or ErbB), ErbB2 (Neu, HER2), ErbB3 (HER3), and ErbB4 (HER4). All family members are type I transmembrane glycoproteins that have an extracellular domain with two ligand binding cysteine rich domains, separated by a spacer region, and a cytoplasmic domain with a membrane proximal tyrosine kinase domain and a C-terminal tail with multiple tyrosine autophosphorylation sites. The human EGF R gene encodes a 1210 amino acid (aa) residue precursor with a 24 aa putative signal peptide, a 621 aa extracellular domain, a 23 aa transmembrane domain, and a 542 aa cytoplasmic domain. EGF R has been shown to bind a subset of the EGF family ligands, including EGF, amphiregulin, TGF alpha , betacellulin, epiregulin, heparin-binding EGF and neuregulin-2 alpha , in the absence of a coreceptor. Ligand binding induces EGF R homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, tyrosine phosphorylation and cell signaling. EGF R can also be recruited to form heterodimers with ligand-activated ErbB3 or ErbB4. EGF R signaling has been shown to regulate multiple biological functions including cell proliferation, differentiation, motility and apoptosis. In addition, EGF R signaling has also been shown to play a role in carcinogenesis (1‑3).

1. Daly, R.J. (1999) Growth Factors, 16:255.
2. Schlessinger, J. (2000) Cell. 103:211.
3. Maihle, N.J. et al. (2002) Cancer Treat. Res. 107:247.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EGFR Antibody (FAB10951F)(5)

Publications using FAB10951F

• Doshi, MB;Lee, N;Tseyang, T;Ponomarova, O;Goel, HL;Spears, M;Li, R;Zhu, LJ;Ashwood, C;Simin, K;Jang, C;Mercurio, AM;Walhout, AJM;Spinelli, JB;Kim, D; Disruption of sugar nucleotide clearance is a therapeutic vulnerability of cancer cells Nature 2023-10-25 [PMID: 37880368] (Flow Cytometry, Human)
• Emira Bousoik, Ramina Nabiee, Farideh Amirrad, Ashley Nichols, Rebecca Witt, Parvin Mahdipoor et al. Heterogeneity and Plasticity of Human Breast Cancer Cells in Response to Molecularly-Targeted Drugs Frontiers in Oncology 2019-10-15 [PMID: 31681603]
• C Stegmann, D Hochdorfer, D Lieber, N Subramania, D Stöhr, K Laib Sampa, C Sinzger A derivative of platelet-derived growth factor receptor alpha binds to the trimer of human cytomegalovirus and inhibits entry into fibroblasts and endothelial cells PLoS Pathog., 2017-04-12;13(4):e1006273. 2017-04-12 [PMID: 28403220] (Flow Cytometry, Human)
• Ana C. Parente-Pereira, Lynsey M. Whilding, Nancy Brewig, Sjoukje J. C. van der Stegen, David M. Davies, Scott Wilkie et al. Synergistic Chemoimmunotherapy of Epithelial Ovarian Cancer Using ErbB-Retargeted T Cells Combined with Carboplatin The Journal of Immunology 2013-09-01 [PMID: 23898037]
• Moore RG, Lange TS, Robinson K Efficacy of a non-hypercalcemic vitamin-D2 derived anti-cancer agent (MT19c) and inhibition of fatty acid synthesis in an ovarian cancer xenograft model. PLoS ONE, 2012-04-03;7(4):e34443. 2012-04-03 [PMID: 22509304] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: EGFR
• Uniprot:
  • CAA25240()