Recombinant Human EGF Protein

Product Discontinued

Recombinant Human EGF Protein Summary

• Description: Recombinant biologically active EGF protein.

  Source:E. coli

• Details of Functionality: Recombinant Human Epidermal Growth Factor is fully biologically active when compared to standards.The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells(measured by 3H-thymidine uptake) is less then 0.1 ng/ml corresponding to a specific activity of 1 x 10^7 Units/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: EGF
• Purity: >95%, by HPLC
• Endotoxin Note: Less than 0.1 ng/ug (IEU/ug) of recombinant EGF .

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 6.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a concentrated solution containing PBS pH-7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by HPLC
• Reconstitution Instructions: Reconstitute with sterilized 18 M omega-cm water to a final concentration of at least 0.1 mg/ml.

Notes

After reconstitution, store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Alternate Names for Recombinant Human EGF Protein

• beta-urogastrone
• EGF
• epidermal growth factor (beta-urogastrone)
• epidermal growth factor
• hEGF
• HOMG4
• pro-epidermal growth factor
• URG
• Urogastrone

Background

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: EGF