Recombinant Human EGF Protein Summary
An un-tagged recombinant protein corresponding to the amino acids 971-1023 of Human EGF
Amino Acid Sequence: MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR
< 1.0 EU per 1 ug of protein (determined by LAL method)
|Details of Functionality
The ED50 for this effect is < 0.89 ng/ml. Measured in a cell proliferation assay using mouse Balb3T3 cell.
| Protein/Peptide Type
Biologically Active Protein
>95% pure by SDS-PAGE
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Phosphate-buffered saline (pH 7.4)
>95% pure by SDS-PAGE
Alternate Names for Recombinant Human EGF Protein
- epidermal growth factor (beta-urogastrone)
- epidermal growth factor
- pro-epidermal growth factor
Recombinant human epidermal growth factor(EGF) is a 6.3 kDa globular protein containing 54 amino acids residues, including 3 intra-molecular disulfide bonds. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. Recombinant EGF was expressed in E.coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 2 years from date of receipt.
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