EGF Antibody

Western Blot: EGF Antibody [NBP1-19806] - Analysis of EGF (R709) pAb in extracts from NIH-3T3 cells.

Immunohistochemistry-Paraffin: EGF Antibody [NBP1-19806] - Human hepatocellular carcinoma (HCC). Antibody at 1:100. Heat/citrate buffer antigen retrieval. Permeabilization with 2% Triton X-100. Counterstained with hematoxylin. IHC-P image submitted by a verified customer review.

Immunohistochemistry: EGF Antibody [NBP1-19806] - Human brain tissue.

Immunohistochemistry-Paraffin: EGF Antibody [NBP1-19806] - Staining of cells surrounding a follicle and the cytoplasm of the follicle using anti-EGF antibody.

Product Discontinued

EGF Antibody Summary

• Immunogen: Synthetic peptide made to an internal portion of the human EGF protein (between amino acids 700-800) [UniProt P01133]
• Localization: Transmembrane Protein with Extracellular, Helical and Cytoplasmic Domains
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EGF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EGF Antibody

• beta-urogastrone
• EGF
• epidermal growth factor (beta-urogastrone)
• epidermal growth factor
• hEGF
• HOMG4
• pro-epidermal growth factor
• URG
• Urogastrone

Background

Epidermal growth factor (EGF) is a 53 amino acid growth factor that stimulates cell growth, proliferation, and differentiation upon binding to its receptor, EGFR. EGF binding to EGFR promotes dimerization of EGFR and phosphorylation on its tyrosine residues. Tyrosine phosphorylation induces a signal cascade that results in the growth of various epidermal and epithelial tissues in vivo and in vitro. EGF is found in most body fluids including as urine (urogastrone), saliva, milk and platelet-rich plasma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EGF Antibody (NBP1-19806)(8)

Publications using NBP1-19806

• Aidoukovitch A, Bodahl S, Tufvesson E, Nilsson BO Desquamated Epithelial Cells of Unstimulated Human Whole Saliva Express Both EGF Transcript and Protein International journal of dentistry 2022-12-17 [PMID: 36573202] (DB, Human)
• da Rocha J, Bastos L, Domingues S et al. APP binds to the EGFR ligands HB-EGF and EGF, acting synergistically with EGF to promote ERK signaling and neuritogenesis Mol Neurobiol 2020-10-03 [PMID: 33009641]
• Guo FF, Xiao M, Wang SY et al. Downregulation of mitogen-activated protein kinases (MAPKs) in chronic ethanol-induced fatty liver Toxicol. Mech. Methods 2020-04-01 [PMID: 32237978]
• Chou Pr, Lin Yn, Wu Sh et Al. Supercritical Carbon Dioxide-decellularized Porcine Acellular Dermal Matrix combined with Autologous Adipose-derived Stem Cells: Its Role in Accelerated Diabetic Wound Healing Int J Med Sci 2020-02-04 [PMID: 32132871] (IHC-P, Rat)
• Lee CY, Yang CY, Lin CC et al. Hair growth is promoted by BeauTop via expression of EGF and FGF-7 Mol Med Rep 2018-06-01 [PMID: 29693180] (IF/IHC, Mouse)
• Ren W, Sun Y, Cheema S, Du Ket al. Interaction of Constitutive Photomorphogenesis 1 Protein with Protein-Tyrosine Phosphatase 1B Suppresses Protein-Tyrosine Phosphatase 1B Activity and Enhances Insulin Signaling. J Biol Chem 2013-04-12 [PMID: 23439647] (Human)
• Bretschi M, Cheng C, Witt H et al. Cilengitide affects tumor compartment, vascularization and microenvironment in experimental bone metastases as shown by longitudinal (18)F-FDG PET and gene expression analysis J Cancer Res Clin Oncol 2012-12-11 [PMID: 23229276] (IF/IHC, Human)
• Sette G, Salvati V, Memeo L et al. EGFR Inhibition Abrogates Leiomyosarcoma Cell Chemoresistance through Inactivation of Survival Pathways and Impairment of CSC Potential PLoS One 2012-01-01 [PMID: 23056514] (IF/IHC, Human)

Reviews for EGF Antibody (NBP1-19806) (3)

Average Rating: 4.3

(Based on 3 reviews)

We have 3 reviews tested in 2 species: Human, Mouse.

reviewed by: Mozhdeh Sojoodi IHC-P Human 06/24/2021

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Liver
• Species: Human
• Lot: A2

Comments

• Comments: Primary antibody dilution: 1:100
  Heat/citrate buffer antigen retrieval
  Permeabilization with 2% TritonX100
  Counter stained with Hematoxylin

reviewed by: Feng Li ICC Mouse 05/17/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: mouse liver
• Species: Mouse
• Lot: lot A1

reviewed by: Joana Rocha IP Human 07/14/2016

Summary

• Application: Immunoprecipitation
• Species: Human
• Lot: A1

FAQs for EGF Antibody (NBP1-19806). (Showing 1 - 1 of 1 FAQs).

1. I recently acquired your anti-EGF antibody ref NBP1-19806. In the datasheet you don't have the indication that it can be used for imunoprecipitation/co-imunoprecipitation. Do you have data proving it does not work? or it was never tested?
   • This antibody has not been tested/validated in Immunoprecipitation. If it was tested and did not work it would be noted in the application section with IP(-).

Bioinformatics

• Gene Symbol: EGF
• Entrez:
  • 1950(Human)
  • 13645(Mouse)
  • 25313(Rat)
• Uniprot:
  • P01133()