EGF Antibody (JA14-15)

Immunohistochemistry-Paraffin: EGF Antibody (JA14-15) [NBP2-66819] - Analysis of paraffin-embedded human skin tissue using anti-EGF antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: EGF Antibody (JA14-15) [NBP2-66819] - Analysis of paraffin-embedded human kidney tissue using anti-EGF antibody. Counter stained with hematoxylin.

Product Discontinued

EGF Antibody (JA14-15) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within human EGF aa 201-350/1,207 (SwissProt: P01133 Human)
• Localization: Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: EGF
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:1000
• Theoretical MW: 130/10 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for EGF Antibody (JA14-15)

• beta-urogastrone
• EGF
• epidermal growth factor (beta-urogastrone)
• epidermal growth factor
• hEGF
• HOMG4
• pro-epidermal growth factor
• URG
• Urogastrone

Background

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EGF