EGF Antibody (F5)

Product Discontinued

EGF Antibody (F5) Summary

• Immunogen: EGF coupled on bovine serum albumin and tetanus toxoide.The antibody reacts with mouse EGF in ELISA and in spot blots.
• Specificity: The antibody reacts with mouse EGF in ELISA and in spot blots. In immunohisto­chemistry the antibody reacts with formalin fixed and paraffin embedded mouse salivary glands. It also reacts with human Brunner's glands (presumably with urogastr­on). Immunogen: EGF coupled on bovine serum albumin and tetanus toxoide.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: EGF
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Western Blot 1:100 - 1:2000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA
• Preservative: 20 mM Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for EGF Antibody (F5)

• beta-urogastrone
• EGF
• epidermal growth factor (beta-urogastrone)
• epidermal growth factor
• hEGF
• HOMG4
• pro-epidermal growth factor
• URG
• Urogastrone

Background

The EGFR is a ubiquitously expressed 180 kD membrane tyrosine kinase that dimerizes upon binding EGF, transforming growth factor alpha, or other ligands. Dimerization initiates downstream signaling that elicits extensive phosphorylation of the receptor resulting in mitogenesis and protection against apoptotic stimuli. EGF receptor expression is amplified in many squamous carcinomas and in the bronchi of high-risk smokers. The receptor is internalized and degraded after ligand binding. EGFR extensively interacts with a number of proteins and is also reported to be a cellular receptor for cytomegalovirus. The Poly6213 antibody recognizes the human, mouse, and rat EGFR and has been shown to be useful for Western blotting. Interacts with Grb2, SHP1 and 2, VAV2 and 3, FAK, c-Src, GAB1, NCK1, Shc, human cytomegalovirus and numerous other proteins

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EGF