EGF Antibody (10827) [Unconjugated]

EGF was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human/Primate EGF Monoclonal Antibody (Catalog # MAB636) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membrane and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu, Pm
• Applications: IHC, ELISA(Cap)
• Clone: 10827
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

EGF Antibody (10827) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human EGF
• Specificity: Detects human and primate EGF in ELISAs. In sandwich ELISAs, no cross-reactivity or interference is observed with recombinant mouse (rm) EGF, bovine FGF acidic, bovine FGF basic, recombinant human (rh) G-CSF, rhGM-CSF, rmGM-CSF, rhLIF, human PDGF, porcine PDGF, rhTGF‑ alpha, hTGF-beta 1, pTGF-beta 1, rhTGF-beta 1, porcine TGF-beta 1.2, porcine TGF-beta 2, rhTNF-alpha, or rhTNF-beta.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: EGF

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Human EGF Biotinylated Antibody (Catalog number BAF236)
  Standard: Recombinant Human EGF (Catalog number 236-EG)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EGF Antibody (10827) [Unconjugated]

• beta-urogastrone
• EGF
• epidermal growth factor (beta-urogastrone)
• epidermal growth factor
• HOMG4
• pro-epidermal growth factor
• URG
• Urogastrone

Background

EGF is the prototypic member of a family of growth factors that are characterized by the presence of EGF like domains and activate members of the EGF receptor family. Proteolytic cleavage of a membrane-bound precursor releases mature soluble EGF which interacts with the EGF R to promote proliferation and differentiation of mesenchymal and epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EGF Antibody (MAB636)(2)

Publications using MAB636

• Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis. J. Immunol. Methods, 2012;378(1):72-80. 2012 [PMID: 22366959] (ELISA Development, Human)
• Wirth S, Segretin ME, Mentaberry A, Bravo-Almonacid F Accumulation of hEGF and hEGF-fusion proteins in chloroplast-transformed tobacco plants is higher in the dark than in the light. J. Biotechnol., 2006;125(2):159-72. 2006 [PMID: 16584796] (Western Blot, Human)

Bioinformatics

• Gene Symbol: EGF