EDNRB/Endothelin R Type B Antibody

Western blot shows lysates of HEK293 human embryonic kidney cell line, A172 human glioblastoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human EDNRB/Endothelin R Type B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4496) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EDNRB/Endothelin R Type B at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

EDNRB/Endothelin R Type B was detected in immersion fixed A172 human glioblastoma cell line using 10 µg/mL Sheep Anti-Human EDNRB/Endothelin R Type B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4496) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

A172 human glioblastoma cell line was stained with Human EDNRB/Endothelin R Type B Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4496, filled histogram) or isotype control antibody (Catalog # 5‑001‑A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

EDNRB/Endothelin R Type B Antibody Summary

• Immunogen: E. coli-derived recombinant human EDNRB
  Glu27-Lys101
  Accession # P24530
• Specificity: Detects human EDNRB in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: EDNRB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EDNRB/Endothelin R Type B Antibody

• ABCDS
• EDNRB
• endothelin B receptor
• Endothelin receptor non-selective type
• endothelin receptor type B
• ETB
• ET-B
• ETBR
• ET-BR
• ETRB
• HSCR
• HSCR2
• WS4A

Background

EDNRB (Endothelin B Receptor) is a member of the beta-family of rhodopsin receptors. It binds endothelin 1, 2, and 3 and is found on endothelial cells where it mediates vasodilation. Mature human EDNRB is a 7-transmembrane glycoprotein that is 416 amino acids (aa) in length. It contains a 75 aa N-terminal extracellular region (aa 27-101), and a 44 aa C-terminal cytoplasmic domain. There are three EDNRB variants that affect aa 27-101. One shows a 90 aa N-terminal extension, a second shows the same 90 aa N-terminal substitution coupled with a deletion of aa 268-398, and a third shows proteolytic cleavage between Arg64-Ser65. Over aa 27-101, human EDNRB shares 67% and 97% aa sequence identity with mouse and canine EDNRB, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EDNRB
• Uniprot:
  • P24530()