EDNRA/Endothelin R Type A Antibody

Images

 
Western Blot: EDNRA/Endothelin R Type A Antibody [NB600-836] - EDN 1 Receptor A in 1 MES Cells. Western Provided by verified customer review.
Immunocytochemistry/ Immunofluorescence: EDNRA/Endothelin R Type A Antibody [NB600-836] - EDN 1 Receptor A in 1 MES Cells. Immunofluorescent staining image provided by verified customer review.
Western Blot: EDNRA/Endothelin R Type A Antibody [NB600-836] - ET-1 A & B Receptor Western provided by verified customer review.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
1.7 mg/ml

Order Details

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Catalog# & Conjugate Size Price

EDNRA/Endothelin R Type A Antibody Summary

Immunogen
Synthetic peptide corresponding to amino acids 410-422 (QEQNHNTERSSHK) of Rat Endothelin A Receptor, conjugated to KLH.
Localization
Plasma membrane
Specificity
This recognizes epitopes on the cytoplasmic side of the receptor and will not block the binding of endothelins. Therefore, the cell membrane must be permeable to the.
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
EDNRA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 10-20 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 20-40 ug/ml
Application Notes
WB usage reported by verified customer review.
Reviewed Applications
Read 1 Review rated 4
using
NB600-836 in the following applications:

Publications
Read Publications using
NB600-836 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.7 mg/ml
Purity
Protein G purified

Alternate Names for EDNRA/Endothelin R Type A Antibody

  • EDNRA
  • endothelin receptor type A
  • endothelin-1 receptor
  • endothelin-1-specific receptor
  • ET1-specific type
  • ETAR
  • ETRA
  • G protein-coupled receptor
  • hET-AR

Background

Endothelins are a family of peptides that are potent vasoconstrictors produced by mammalian vascular endothelial cells. The receptors for endothelins are widely expressed on tissues. This sheep antibody recognizes epitopes on the cytoplasmic region of the ET(A) Receptor. The binding of this antibody does not interfere with the function of the receptor so that endothelins can interact with the receptor while the antibody is present.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EDNRA/Endothelin R Type A Antibody (NB600-836)(3)

Review for EDNRA/Endothelin R Type A Antibody (NB600-836) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB600-836:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot EDNRA/Endothelin R Type A NB600-836
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reviewed by:
Anonymous
WB Mouse 02/11/2014
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Summary

ApplicationWestern Blot
Sample Testedmesangial cell lysate glomerular lysate
SpeciesMouse
Comments10 % gelmulti pass protein 65 degrees 1 to overnight to aviod aggregates

Blocking

Blocking Details5 % milk pbst

Primary Anitbody

Dilution Ratio1: 2000 on primary

Secondary Antibody

Secondary Descriptionanti sheep hrp
Secondary Concentration1 : 5000 2 secondary

Details

Detection Notesfully wet transfer

Comments

Comments10 % gelmulti pass protein 65 degrees 1 to overnight to aviod aggregates

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EDNRA/Endothelin R Type A Antibody (NB600-836) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 650 Labeled NB600-836C

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Bioinformatics Tool for EDNRA/Endothelin R Type A Antibody (NB600-836)

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Diseases for EDNRA/Endothelin R Type A Antibody (NB600-836)

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Recent Reviews

4
5
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4
1
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2
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1
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Anonymous
02/11/2014
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol EDNRA
Entrez