EDC4 Antibody - BSA Free

Western Blot: EDC4 Antibody [NBP2-16267] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 5% SDS PAGE gel, diluted at 1:2000.

Immunocytochemistry/Immunofluorescence: EDC4 Antibody [NBP2-16267] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EDC4 protein stained by EDC4 antibody diluted at 1:500. Blue: Hoechst 33343 staining.

Immunohistochemistry-Paraffin: EDC4 Antibody [NBP2-16267] - EDC4 antibody detects EDC4 protein at cytosol and nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. EDC4 antibody dilution: 1:500.

Western Blot: EDC4 Antibody [NBP2-16267] - Sample (30 ug of whole cell lysate) A: MCF-7 5% SDS PAGE gel, diluted at 1:3000.

Immunohistochemistry-Paraffin: EDC4 Antibody [NBP2-16267] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:100 dilution.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

EDC4 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human EDC4. The exact sequence is proprietary.
• Localization: Cytoplasm; P-body; Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EDC4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 152 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for EDC4 Antibody - BSA Free

• Autoantigen Ge-1
• Autoantigen RCD-8
• enhancer of mRNA decapping 4
• enhancer of mRNA-decapping protein 4
• Ge-1
• Hedls
• HEDLSHuman enhancer of decapping large subunit
• RCD-8

Background

In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: EDC4
• Uniprot:
  • Q6P2E9(Human)