Dcp1a Antibody (3G4)

Images

 
Western Blot: Dcp1a Antibody (3G4) [H00055802-M06] - Western Blot analysis of DCP1A expression in IMR-32 ( Cat # L008V1 ).
Immunocytochemistry/ Immunofluorescence: Dcp1a Antibody (3G4) [H00055802-M06] - Analysis of monoclonal antibody to DCP1A on HeLa cell. Antibody concentration 10 ug/ml
ELISA: Dcp1a Antibody (3G4) [H00055802-M06] - Detection limit for recombinant GST tagged DCP1A is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clone
3G4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Dcp1a Antibody (3G4) Summary

Immunogen
DCP1A (NP_060873, 186 a.a. ~ 286 a.a) partial recombinant protein with GST tag.STQLSNLGSTETLEEMPSGSQDKSAPSGHKHLTVEELFGTSLPKEQPAVVGLDSEEMERLPGDASQKEPNSFLPFPFEQLGGAPQSETLGVPSAAHHSVQ
Specificity
DCP1A - DCP1 decapping enzyme homolog A (S. cerevisiae)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DCP1A
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA. Use in immunprecipitation reported in scientific literature (PMID 21883093)
Reviewed Applications
Read 1 Review rated 5
using
H00055802-M06 in the following applications:

Publications
Read Publications using
H00055802-M06 in the following applications:

  • 2 publications
  • IP
    1 publication
  • WB
    4 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25530357).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Dcp1a Antibody (3G4)

  • DCP1 decapping enzyme homolog A (S. cerevisiae)
  • decapping enzyme hDcp1a
  • EC 3.-
  • FLJ21691
  • mRNA-decapping enzyme 1A
  • Nbla00360
  • putative protein product of Nbla00360
  • Smad4-interacting transcriptional co-activator
  • SMAD4IP1
  • SMIFHSA275986
  • Transcription factor SMIF

Background

Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dcp1a Antibody (H00055802-M06)(37)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(2)
IP
(1)
WB
(4)
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Human
(7)
Mouse
(1)
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Showing Publications 1 - 10 of 37. Show All 37 Publications.
Publications using H00055802-M06 Applications Species
Kato Y, Katsuki T, Kokubo H et al. Dazl is a target RNA suppressed by mammalian NANOS2 in sexually differentiating male germ cells. Nat Commun 2016 Apr 13 [PMID: 27072294] (WB) WB
Amadei G, Zander MA, Yang G et al. A Smaug2-Based Translational Repression Complex Determines the Balance between Precursor Maintenance versus Differentiation during Mammalian Neurogenesis. J Neurosci. 2015 Nov 25 [PMID: 26609159]
Seto E, Onizuka Y, Nakajima-Shimada J. Host cytoplasmic processing bodies assembled by Trypanosoma cruzi during infection exert anti-parasitic activity. Parasitol Int. 2015 Jul 30 [PMID: 26232634]
Ghosh S, Bose M, Ray A et al. Polysome arrest restricts miRNA turnover by preventing exosomal export of miRNA in growth-retarded mammalian cells. Mol Biol Cell. 2015 Mar 15 [PMID: 25609084] (WB, Human) WB Human
Terrell AM, Anand D, Smith SF et al. Molecular characterization of mouse lens epithelial cell lines and their suitability to study RNA granules and cataract associated genes. Exp. Eye Res. 2014 Dec 19 [PMID: 25530357] (ICC/IF, Mouse) ICC/IF Mouse
Perez-Vilaro G, Fernandez-Carrillo C, Mensa L et al. Hepatitis C virus infection inhibits P-body granule formation in human livers. J Hepatol. 2014 Nov 06 [PMID: 25463546]
Seto E, Inoue T, Nakatani Y et al. Processing bodies accumulate in human cytomegalovirus-infected cells and do not affect viral replication at high multiplicity of infection. Virology. 2014 Jun [PMID: 24928047]
Ling Y H, Wong C C, Li K W et al. CCHCR1 interacts with EDC4, suggesting its localization in P-bodies. Exp Cell Res. 2014 Sep 10 [PMID: 24858563] (Human) Human
Kaehler C, Isensee J, Hucho T et al. 5-Fluorouracil affects assembly of stress granules based on RNA incorporation. Nucleic Acids Res. 2014 Apr 11 [PMID: 24728989]
Li Y, Masaki T, Shimakami T, Lemon SM. hnRNP L and NF90 interact with hepatitis C virus 5' terminal untranslated RNA and promote efficient replication. J Virol. 2014 Apr 09 [PMID: 24719423]
Show All 37 Publications.

Review for Dcp1a Antibody (H00055802-M06) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mus Musculus.

Reviews using H00055802-M06:
Filter by Applications
ICC
(1)
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Filter by Species
Mus Musculus
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Dcp1a H00055802-M06
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reviewed by:
Anonymous
ICC Mus Musculus 03/16/2018
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Summary

ApplicationImmunocytochemistry
Sample TestedRAW264.7 cells
SpeciesMus Musculus
LotGB242-3G4

Comments

CommentsIF analysis of Dcp1a protein with antibody at 1:100 dilution in RAW cell lines using a Nikon Widefield Fluorescence microscope

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Dcp1a Antibody (H00055802-M06) (0)

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Recent Reviews

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5
1
4
0
3
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2
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1
0

Anonymous
03/16/2018
Application: ICC
Species: Mus Musculus

Bioinformatics

Gene Symbol DCP1A
Entrez
OMIM
Uniprot