Pyruvate Dehydrogenase E2 Antibody - BSA Free

Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-34065] - Analysis using Anti-DLAT antibody NBP2-34065 (A) shows similar pattern to independent antibody NBP2-56744 (B).

Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase E2 Antibody [NBP2-34065] - Staining of human cell line U-2 OS shows positivity in mitochondria. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-34065] - Staining of human liver shows low expression as expected

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-34065] - Staining of human heart muscle shows high expression.

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-34065] - Staining of human rectum shows strong cytoplasmic granular positivity in glandular cells.

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-34065] - Staining of human placenta shows strong cytoplasmic granular positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-34065] - Staining of human kidney shows strong cytoplasmic granular positivity in cells in tubules.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Pyruvate Dehydrogenase E2 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: LLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILVPEGTRDVPLGTPLCIIVEKEADISAFADYRPTEVTDLKPQVPPPT
• Predicted Species: Mouse (91%), Rat (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DLAT
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Pyruvate Dehydrogenase E2 Recombinant Protein Antigen (NBP2-34065PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Pyruvate Dehydrogenase E2 Antibody - BSA Free

• 70 kDa mitochondrial autoantigen of primary biliary cirrhosis
• dihydrolipoamide S-acetyltransferase
• DLTA
• E2 component of pyruvate dehydrogenase complex
• EC 2.3.1
• M2 antigen complex 70 kDa subunit
• PBC
• PDC-E2EC 2.3.1.12
• PDCE2mitochondrial

Background

The DLAT gene encodes dihydrolipoamide acetyltransferase (EC 2.3.1.12), the E2 subunit of the mammalian pyruvate dehydrogenase complex (PDC; EC 1.2.4.1) of the inner mitochondrial membrane. Patients with primary biliary cirrhosis (PBC; MIM 109720) show autoantibodies to DLAT.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pyruvate Dehydrogenase E2 Antibody (NBP2-34065)(1)

Publication using NBP2-34065

• Kam, J H, Weinrich, T W Et al. Mitochondrial absorption of short wavelength light drives primate blue retinal cones into glycolysis which may increase their pace of aging. Vis Neurosci 2019-01-01 [PMID: 31199213] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: DLAT
• Uniprot:
  • P10515(Human)