ECT2 [p Thr790] Antibody - BSA Free

Western Blot: ECT2 [p Thr790] Antibody [NBP1-77939] - Shows detection of endogenous phosphorylated ECT2 (arrowhead) present in cell lysates from interphase (lane 1) and mitotic (lane 2) HeLa cells. Despite specific staining of interphase cells, this reagent is believed to be phospho specific based on ELISA results using both phosphorylated and non-phosphorylated immunizing peptide. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000. Personal Communication, Toru Miki, CCR-NCI, Bethesda, MD.

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ECT2 [p Thr790] Antibody - BSA Free Summary

• Description: This affinity-purified antibody is directed against the phosphorylated form of human ECT2 protein at the [p Thr790] residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This affinity purified ECT2 [p Thr790] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ECT2 protein. (Uniprot: Q9H8V3)
• Modification: p Thr790
• Specificity: This affinity-purified antibody is directed against the phosphorylated form of human ECT2 protein at the pT790 residue. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human ECT2 protein at the pT790 residue. By ELISA the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human ECT2 is minimal by ELISA, but western blot shows specific staining of interphase cell lysates, which are predicted to contain non-phosphorylated ECT2. Additional experiments are underway to clarify antibody specificity. A BLAST analysis was used to suggest cross reactivity with ECT2 protein from human, mouse, rat, zebrafish, chimpanzee, chicken, and dog based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ECT2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000-1:40000
  • Immunoprecipitation
  • Western Blot 1:1000-1:4000
• Application Notes: This affinity-purified antibody has been tested for use in ELISA, western blot and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 101 kDa in size by western blotting in the appropriate cell lysate or extract. Less than 2% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is believed to be phospho specific for ECT2 phosphorylated at the pT790 residue. Experiments to generate more conclusive evidence are currently under preparation.

Reactivity Notes

A BLAST analysis was used to suggest cross reactivity with ECT2 protein from human, mouse, rat, zebrafish, chimpanzee, chicken, and dog based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ECT2 [p Thr790] Antibody - BSA Free

• ARHGEF31
• epithelial cell transforming sequence 2 oncogene
• Epithelial cell-transforming sequence 2 oncogene
• FLJ10461
• MGC138291
• protein ECT2

Background

ECT2 is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of ECT2 is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ECT2
• Uniprot:
  • NP_060568(Human)
  • Q9H8V3(Human)