ECT2 [p Thr790] Antibody

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Western Blot: ECT2 [p Thr790] Antibody [NBP1-77939] - Shows detection of endogenous phosphorylated ECT2 (arrowhead) present in cell lysates from interphase (lane 1) and mitotic (lane 2) HeLa cells. Despite specific ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Ch, Pm, ZeSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ECT2 [p Thr790] Antibody Summary

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 785-795 of human ECT2 protein.
Modification
p Thr790
Specificity
This antibody is directed against the phosphorylated form of human ECT2 protein at the pT790 residue.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ECT2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:4000
  • ELISA 1:10000-1:40000
Application Notes
This affinity-purified antibody has been tested for use in ELISA, western blot and immunoprecipitation. Expect a band approximately 101 kDa in size by western blotting in the appropriate cell lysate or extract. Less than 2% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is believed - be phospho specific for ECT2 phosphorylated at the pT790 residue. Experiments - generate more conclusive evidence are currently under preparation.

Reactivity Notes

Reactive in human, mouse, rat, zebrafish, chimpanzee, chicken, dog

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ECT2 [p Thr790] Antibody

  • ARHGEF31
  • epithelial cell transforming sequence 2 oncogene
  • Epithelial cell-transforming sequence 2 oncogene
  • FLJ10461
  • MGC138291
  • protein ECT2

Background

ECT2 is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of ECT2 is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ECT2
Uniprot