ECSIT Antibody - BSA Free

Western Blot: ECSIT Antibody [NBP1-76594] - Human heart cell lysates with ECSIT antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.

Immunohistochemistry: ECSIT Antibody - BSA Free [NBP1-76594] - Immunohistochemistry of ECSIT in mouse heart cells with ECSIT antibody at 2 ug/mL.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ECSIT Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ECSIT Antibody - BSA Free (NBP1-76594) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human ECSIT. The immunogen is located within amino acids 290 - 340 of ECSIT. Amino Acid Squence: PPEEREVEETPEEW
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ECSIT
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Paraffin 2 ug/ml
  • Western Blot 0.5-2 ug/ml
• Control Peptide:

  ECSIT Peptide (NBP1-76594PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for ECSIT Antibody - BSA Free

• ECSIT homolog (Drosophila)
• evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
• likely ortholog of mouse signaling intermediate in Toll pathway evolutionarilyconserved
• Protein SITPEC
• signaling intermediate in Toll pathway evolutionarily conserved ortholog
• SITPECsignaling intermediate in Toll pathway, evolutionarily conserved

Background

Activation of NF-kappaB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 to kinases such as IRAK and other intermediates such as TNF receptor associated factor (TRAF)-6. ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway. Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis, indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ECSIT
• Uniprot:
  • NP_057665(Human)