ECE-1 Antibody (303908)

• Reactivity: Hu
• Applications: WB
• Clone: 303908
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

ECE-1 Antibody (303908) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ECE-1
  Gln90-Trp770
  Accession # P42892
• Specificity: Detects human ECE-1 in direct ELISAs and Western blots. Does not cross‑react with recombinant human (rh) ECE-2 or rhMMEL-2.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: ECE1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ECE-1 Antibody (303908)

• EC 3.4.24
• EC 3.4.24.71
• ECE
• ECE1
• ECE-1
• endothelin converting enzyme 1
• endothelin-converting enzyme 1

Background

Endothelin-converting Enzyme 1 (ECE-1) is a zinc protease of the neprilysin (NEP) family, which also includes ECE-2, PEX, XCE, DINE, Kell and several NEP-like proteins (1). ECE-1 is a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Four alternatively spliced isoforms differ in their cytoplasmic tail (2, 3). In addition to big endothelin-1, ECE-1 cleaves a variety of bioactive peptides such as bradykinin, neurotensin, angiotensin I, and substance P (1). Together with ECE-2, it is also involved in degradation of beta -amyloid peptide (4). The ectodomain of human ECE-1, which is common to all isoforms, was expressed with an N-terminal His tag and purified.

1. Turner, A.J. et al. (2001) BioEssays 23:261.
2. Valdennaire, O. et al. (1999) Eur. J. Biochem. 264:341.
3. Schweizer, A. et al. (1997) Biochem. J. 328:871.
4. Eckman, E.A. et al. (2003) J. Biol. Chem. 278:2081.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ECE-1 Antibody (MAB1784)(1)

Publication using MAB1784

• Hein TW, Ren Y, Yuan Z, Xu W, Somvanshi S, Nagaoka T, Yoshida A, Kuo L Functional and molecular characterization of the endothelin system in retinal arterioles. Invest. Ophthalmol. Vis. Sci., 2009-01-17;50(7):3329-36. 2009-01-17 [PMID: 19151386] (Western Blot, Porcine)

Bioinformatics

• Gene Symbol: ECE1
• Uniprot:
  • P42892()