ECE-2 Antibody (233914) [Unconjugated]

ECE-2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 3 µg/mL Rat Anti-Human ECE-2 Monoclonal Antibody (Catalog # MAB16451) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 233914
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

ECE-2 Antibody (233914) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ECE-2
  Val104-Leu787
  Accession # O60344
• Specificity: Detects human ECE-2 in direct ELISAs and Western blots. In Western blots, 30% cross-reactivity with recombinant human (rh) ECE‑1 and no cross-reactivity with rhKELL is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: ECE2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ECE-2 Antibody (233914) [Unconjugated]

• EC 3.4.24
• EC 3.4.24.71
• ECE2
• ECE-2
• endothelin converting enzyme 2
• endothelin-converting enzyme 2
• KIAA0604MGC2408
• MGC17664
• MGC78487

Background

ECE-2 is a zinc protease of the neprilysin (NEP) family, which also includes ECE-1, PEX, XCE, DINE, Kell and several NEP-like proteins (1). ECE-2 is a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Three alternatively spliced forms differ in their cytoplasmic tail (2). In addition to big endothelin-1, ECE-2 cleaves a variety of bioactive peptides such as bradykinin, neurotensin, angiotensin I, substance P, dynorphin B, proenkephalin-derived peptides such as peptide E, BAM 22 and BAM18, and PEN-LEN, an endogenous inhibitor of prohormone convertase 1 (3). Together with ECE-1, it is also involved in degradation of beta -amyloid peptide (4). The ectodomain of human ECE-2 was expressed with an N-terminal His tag and purified.

1. Turner, A.J. et al. (2001) BioEssays 23:261.
2. Lorenzo, M.N. et al. (2001) Biochim. Biophys. Acta 1522:46.
3. Mzhavia, N. et al. (2003) J. Biol. Chem. 278:14704.
4. Eckman, E.A. et al. (2003) J. Biol. Chem. 278:2081.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ECE-2 Antibody (MAB16451)(1)

Publication using MAB16451

• Palmer JC, Baig S, Kehoe PG, Love S Endothelin-converting enzyme-2 is increased in Alzheimer's disease and up-regulated by Abeta. Am. J. Pathol., 2009-06-18;175(1):262-70. 2009-06-18 [PMID: 19541930] (ELISA Development, IHC-P, Human)

Review for ECE-2 Antibody (MAB16451) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer ELISA Human 02/10/2015

Summary

• Application: ELISA
• Sample Tested: See PMID 19541930
• Species: Human

Bioinformatics

• Gene Symbol: ECE2
• Uniprot:
  • O60344()