MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')
2Secondary Antibody (Catalog #
F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox">

MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')
2Secondary Antibody (Catalog #
F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." alt="MCF‑7 human breast cancer cell line was stained with Rat Anti-Human ECE‑1 Monoclonal Antibody (Catalog # MAB17841, filled histogram) or isotype control antibody (Catalog #
MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')
2Secondary Antibody (Catalog #
F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />