Recombinant Mouse EBI3 Protein

Product Discontinued

Recombinant Mouse EBI3 Protein Summary

• Description: Recombinant bioactive protein containing 207 amino acids for Mouse IL-27/IL-35 EBI3 Subunit
  
  

  Source:E. coli
  Amino Acid Sequence:(Q3U1K3)

• Details of Functionality: Assay data for mouse recombinant EBI-3 is based upon qualitative binding to anti-EBI-3 antibody.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: EBI3
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: <0.01 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 22.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 10 mM sodium citrate (pH 3)
• Concentration: Lyoph
• Purity: >90%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute with sterile H2O to 0.1 - 0.5 mg/ml, which can be further diluted into other aqueous solutions.

Alternate Names for Recombinant Mouse EBI3 Protein

• cytokine receptor
• EBI3
• EBI-3
• EBV-induced gene 3 protein
• Epstein-Barr virus induced 3
• Epstein-Barr virus induced gene 3
• Epstein-Barr virus-induced gene 3 protein
• IL-27 beta
• IL-27 subunit beta
• IL27B
• IL-27B
• IL35 EBI3
• IL-35 EBI3
• interleukin-27 subunit beta

Background

Epstein-Barr Virus Induced Gene-3 (EBI-3), is a secreted glycoprotein belonging to the hematopoietin receptor family and related to the p40 subunit of IL-12. It was identified by its induced expression in B-lymphocytes in response to Epstein-Barr virus infection. EBI-3 forms heterodimers with p28 to form IL-27 and with p35 to form IL-35. Both IL-27 and IL-35 have anti-inflammatory and regulatory activity. Recombinant Mouse EBI is a non-glycosylated polypeptide chain consisting of 207 amino acids with a molecular weight of 22,900 Da.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for EBI3 Recombinant Protein (NBP1-99327)(1)

Publication using NBP1-99327

• Mizoguchi I, Ohashi M, Hasegawa H et al. EBV-induced gene 3 augments IL-23Ralpha protein expression through a chaperone calnexin J. Clin. Invest. 2020-08-18 [PMID: 32809973]

Bioinformatics

• Gene Symbol: EBI3