EBI3 Antibody

Western Blot: IL-27/IL-35 EBI3 Subunit Antibody [NBP2-27359] - Analysis using EBI3 antibody. Recombinant mouse EBI3 probed with EBI3 antibody at 5 ug/ml.

Product Discontinued

EBI3 Antibody Summary

• Immunogen: Amino acids 51-77 of mouse EBI3 were used as the immunogen for this antibody.
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EBI3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 3-5ug/ml

Reactivity Notes

The amino acid sequence used as immunogen for this antibody is 100% homologous in mouse and rat and 85% homologous in human.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for EBI3 Antibody

• cytokine receptor
• EBI3
• EBI-3
• EBV-induced gene 3 protein
• Epstein-Barr virus induced 3
• Epstein-Barr virus induced gene 3
• Epstein-Barr virus-induced gene 3 protein
• IL-27 beta
• IL-27 subunit beta
• IL27B
• IL-27B
• IL35 EBI3
• IL-35 EBI3
• interleukin-27 subunit beta

Background

EBI3 is a 228 amino acid containing pro- and anti-inflammatory cytokine belonging to the type I cytokine receptor family and contains two fibronectin type-III domain. It forms a heterodimer with IL27 and is also a component of the IL-12 heterodimer. A protein involved in regulated cell-mediated immune responses, EBI3, has an important role in regulating T helper cell development, suppressing T-cell proliferation, stimulating cytotoxic T cell activity, inducing isotype switching in B-cells, and has diverse effects on innate immune cells. Target cells of EBI3 include CD4 T helper cells, type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. Role of EBI3 is important in antitumor as well as antiangiogenic activity with activation and production of antiangiogenic chemokines. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EBI3