EBI3 Antibody

Western Blot: EBI3 Antibody [NBP2-03937] - Analysis of EBI3 using NBP2-03937 in 1) Daudi cell lysate using 2.0 ug/ml, 2) Ramos cell lysate at 2.0 ug/ml in the absence of immunizing peptide 3) Ramos cell lysate at 2.0 ug/ml in the presence of immunizing peptide and 4) RAW cell lysate at 5.0 ug/ml.

Product Discontinued

EBI3 Antibody Summary

• Immunogen: A portion of amino acids 50-100 of human EBI3 was used as the immunogen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EBI3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2-6 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for EBI3 Antibody

• cytokine receptor
• EBI3
• EBI-3
• EBV-induced gene 3 protein
• Epstein-Barr virus induced 3
• Epstein-Barr virus induced gene 3
• Epstein-Barr virus-induced gene 3 protein
• IL-27 beta
• IL-27 subunit beta
• IL27B
• IL-27B
• IL35 EBI3
• IL-35 EBI3
• interleukin-27 subunit beta

Background

EBI3 is a novel hematopoietin receptor family member related to the p40 subunit of interleukin-12 and to the ciliary neurotrophic factor receptor. It's expression is induced in B lymphocytes by Epstein-Barr virus (EBV) infection. Human EBI3 gene maps to chromosome 19p13.3, near genes encoding the erythropoietin receptor and the cytokine receptor-associated kinase, Tyk2. The protein is expressed in vivo by scattered cells in interfollicular zones of tonsil tissue, by cells associated with sinusoids in perifollicular areas of spleen tissue, and at very high levels by placental syncytiotrophoblasts. EBI3 expression in vitro is induced in EBV-negative cell lines by expression of the EBV latent infection membrane protein-1 and in peripheral blood mononuclear cells by pokeweed mitogen stimulation. The protein plays a crucial role in regulating cell-mediated immune responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EBI3
• Uniprot:
  • Q14213(Human)