EBI3 Antibody

Western Blot: EBI3 Antibody [NBP2-03935] - analysis of EBI3 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide at 2 ug/ml and 3) recombinant human IL-35 protein at 5 ug/ml using EBI3 antibody.

• Reactivity: Hu, Pm
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

EBI3 Antibody Summary

• Immunogen: A portion of amino acids 120-170 of human EBI3 was used as the immunogen
• Specificity: EBI3 is routinely seen in western blot at approximately 35 kD. The amino acid sequence used as immunogen is 100% homologous in human (isoform CRA_a and CRA_b),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EBI3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2-5 ug/ml

Reactivity Notes

Reacts with Chimpanzee. Predicted to cross react with New World Monkey.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for EBI3 Antibody

• cytokine receptor
• EBI3
• EBI-3
• EBV-induced gene 3 protein
• Epstein-Barr virus induced 3
• Epstein-Barr virus induced gene 3
• Epstein-Barr virus-induced gene 3 protein
• IL-27 beta
• IL-27 subunit beta
• IL27B
• IL-27B
• IL35 EBI3
• IL-35 EBI3
• interleukin-27 subunit beta

Background

EBI3 is a 229 amino acid cytokine belonging to the type I cytokine receptor family (type 3 subfamily) with a signal peptide, 2 potential N-linked glycosylation sites, an LSDWS motif, 2 fibronectin type-III domains. It is usually induced due to infection of B lymphocytes by Epstein-Barr virus and due to expression of an EBV encoded latent membrane protein. EBI3 is a subunit of two heterodimeric cytokines interleukin-27 (IL27) and IL35. It is a secreted glycoprotein associated with calnexin and is a heterodimer with an alpha and a beta subunit. It is also known to interact with SQSTM1 and is responsible for the rapid clonal expansion of naive but not memory CD4 (+) T-cells. It strongly synergizes with IL-12 and trigger IFN-gamma production of naive CD4 (+) T-cells. It mediates its biologic effects through the cytokine receptor WSX-1/TCCR. It triggers signaling in T cells, B cells, and myeloid cells and along with p35 it acts as an important immunomodulator. It is usually expressed in various hemopoietic tissues and cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EBI3
• Uniprot:
  • Q14213(Human)