EBI3 Antibody Pack

Western Blot: EBI3 Antibody Pack [NBP2-29379] - Testing of mouse EBI3 monoclonal antibody at A) 0.1 ug/ml on recombinant protein and B) 3 ug/ml on recombinant mouse IL-35 protein. Goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: EBI3 Antibody Pack [NBP2-29379] - Analysis of EBI3 in formalin-fixed, paraffin-embedded mouse brain tissue using an isotype control (top left) and anti-EBI3 antibody (bottom left, right) at 5 ug/ml.

Flow (Intracellular): EBI3 Antibody Pack [NBP2-29379] - Figure A: Intracellular flow cytometric analysis of mouse IL-35 in transfected and untransfected HEK 293 cells (Brefeldin A treated, 5 hours) using anti-mouse EBI3 antibody at 3 ug/10^6 cells. Figure B: Intracellular flow cytometric analysis of mouse EBI3 in stimulated and unstimulated RAW cells (LPS treated, 50 ng/ml, overnight) using anti-mouse EBI3 antibody at 1.5 ug/10^6 cells.

Product Discontinued

EBI3 Antibody Pack Summary

• Immunogen: See individual datasheets.
• Gene: EBI3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular)
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: See individual datasheets for validated applications.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Purity: Immunogen affinity purified

Notes

This pack contains 1 vial each of: NBP2-03935SS (0.025 mg), NBP2-03937SS (0.025 mg), NBP2-03938SS (0.025 mg), NBP2-03939SS (0.025 mg), NBP2-03940SS (0.025 mg), NBP2-03941SS (0.025 mg), NBP2-03944SS (0.025 mg).

Alternate Names for EBI3 Antibody Pack

• cytokine receptor
• EBI3
• EBI-3
• EBV-induced gene 3 protein
• Epstein-Barr virus induced 3
• Epstein-Barr virus induced gene 3
• Epstein-Barr virus-induced gene 3 protein
• IL-27 beta
• IL-27 subunit beta
• IL27B
• IL-27B
• IL35 EBI3
• IL-35 EBI3
• interleukin-27 subunit beta

Background

EBI3 (Epstein-Barr virus Induced-3) is a secreted glycoprotein of the hematopoietin receptor family. It plays a critical regulatory role in the induction of Th2-type immune responses and the development of Th2-mediated tissue inflammation in vivo, which may be mediated through the control of iNKT cell function. EBI3 dimerizes with p28 and p35 subunits of IL-12 to form new proteins IL-27 and IL-35, respectively (Honglian Tong et al, 2010). IL-27 is an early product of activated antigen presenting cell that is produced upon TLR ligation. It negatively regulates Th17 cell differentiation. EBI3 is widely expressed and its expression in dendritic cell is transcriptionally regulated by TLR signaling via MyD88 and NF-kappaB during innate immune responses preceding cytokine driven Th cell development. EBI3 signal inhibits delayed-type hypersensitivity responses by suppressing IL-17 production and inducing IL-10 hyperproduction. EBI3 may play a novel role in controlling tumor metastasis via lung CD8+ T cells, and its deficiency is associated with a diminished production of Th2 cytokines which are known to regulate allergic airway inflammation in asthma. Targeted deletion of EBI3 protects mice from lung metastasis (Kerstin A et al, 2008). Following are the different antibodies included in this EBI3 Sampler Kit:

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EBI3