EBI3 Antibody (355022)

Western blot shows lysates of CHO Chinese hamster ovary cell line either mock transfected or transfected with mouse EBI3. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse IL-27/IL-35 EBI3 Subunit Monoclonal Antibody (Catalog # MAB18341) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-27/IL-35 EBI3 Subunit at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clone: 355022
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

EBI3 Antibody (355022) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL‑27 heterodimer
  Tyr19-Pro228 (EBI3) & Phe29-Ser234 (p28)
  Accession # O35228 (EBI3) & Q8K3I6 (p28)
• Specificity: Detects mouse IL-27/IL-35 EBI3 Subunit in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human IL‑27/IL-35 EBI3 subunit.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: EBI3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EBI3 Antibody (355022)

• cytokine receptor
• EBI3
• EBI-3
• EBV-induced gene 3 protein
• Epstein-Barr virus induced 3
• Epstein-Barr virus induced gene 3
• Epstein-Barr virus-induced gene 3 protein
• IL-27 beta
• IL-27 subunit beta
• IL27B
• IL-27B
• IL35 EBI3
• IL-35 EBI3
• interleukin-27 subunit beta

Background

IL-27 is a heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI3 (Epstein-Barr virus-induced gene 3). IL-27 is expressed by monocytes, endothelial cells and dendritic cells. It binds TCCR/WSX-1 on naive CD4⁺ T cells and induces the expression of a functional IL-12 receptor, making these cells sensitive to IL-12-mediated Th1 cell development. Human EBI3 also associates with the p35 subunit of IL-12 to form IL-35 which is important for immunosuppressive Treg cell induction. EBI3 is 61% amino acid (aa) identical to mouse EBI3 and includes an 20 aa signal peptide anda 209 aa mature protein with two fibronectin type III domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EBI3 Antibody (MAB18341)(8)

Publications using MAB18341

• Dwyer, LJ;Maheshwari, S;Levy, E;Poznansky, MC;Whalen, MJ;S�rbulescu, RF; B cell treatment promotes a neuroprotective microenvironment after traumatic brain injury through reciprocal immunomodulation with infiltrating peripheral myeloid cells Journal of neuroinflammation 2023-05-31 [PMID: 37259118] (Flow Cytometry, Mouse)
• Z Luo, C Soläng, R Larsson, K Singh Interleukin-35 Prevents the Elevation of the M1/M2 Ratio of Macrophages in Experimental Type 1 Diabetes International Journal of Molecular Sciences, 2022-07-19;23(14):. 2022-07-19 [PMID: 35887317] (Flow Cytometry, Mouse)
• Seon-Yeong Lee, Su-Jin Moon, Young-Mee Moon, Hyeon-Beom Seo, Jun-Geol Ryu, A Ram Lee et al. A novel cytokine consisting of the p40 and EBI3 subunits suppresses experimental autoimmune arthritis via reciprocal regulation of Th17 and Treg cells Cellular & Molecular Immunology 2022-01-01 [PMID: 34782759] (ELISA Capture, Human, Mouse)
• SJ Ruiz-Torre, JR Bourn, NM Benight, BG Hunt, C Lester, SE Waltz Macrophage-mediated RON signaling supports breast cancer growth and progression through modulation of IL-35 Oncogene, 2021-11-06;0(0):. 2021-11-06 [PMID: 34743208] (Immunodepletion, Mouse)
• B Min, D Kim, MJ Feige IL-30� (IL-27A): a familiar stranger in immunity, inflammation, and cancer Experimental & Molecular Medicine, 2021-05-28;53(5):823-834. 2021-05-28 [PMID: 34045653] (ELISA Capture, Mouse)
• Kochetkova I, Thornburg T, Callis G, Holderness K, Maddaloni M, Pascual D Oral Escherichia coli colonization factor antigen I fimbriae ameliorate arthritis via IL-35, not IL-27. J Immunol, 2013-12-11;192(2):804-16. 2013-12-11 [PMID: 24337375] (ELISA Development, Mouse)
• Kochetkova I, Golden S, Holderness K IL-35 stimulation of CD39+ regulatory T cells confers protection against collagen II-induced arthritis via the production of IL-10. J. Immunol., 2010-05-10;184(12):7144-53. 2010-05-10 [PMID: 20483737] (Western Blot, Mouse)
• Ilarregui JM, Croci DO, Bianco GA, Toscano MA, Salatino M, Vermeulen ME, Geffner JR, Rabinovich GA Tolerogenic signals delivered by dendritic cells to T cells through a galectin-1-driven immunoregulatory circuit involving interleukin 27 and interleukin 10. Nat. Immunol., 2009-08-09;10(9):981-91. 2009-08-09 [PMID: 19668220] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: EBI3
• Uniprot:
  • O35228 (EBI3) & Q8K3I6 (p28)()