Rev-erb A alpha/NR1D1 Antibody (4F6)

Images

 
Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - NR1D1 monoclonal antibody (M02), clone 4F6 Analysis of NR1D1 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Analysis of monoclonal antibody to NR1D1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Analysis of monoclonal antibody to NR1D1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.
Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Analysis of NR1D1 expression in transfected 293T cell line by NR1D1 monoclonal antibody (M02), clone 4F6. Lane 1: NR1D1 transfected lysate (66.8 KDa). ...read more
Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Analysis of NR1D1 over-expressed 293 cell line, cotransfected with NR1D1 Validated Chimera RNAi ( Cat # H00009572-R01V ) (Lane 2) or non-transfected ...read more
Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - NR1D1 monoclonal antibody (M02), clone 4F6. Analysis of NR1D1 expression in NIH/3T3.
Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - analysis of NR1D1 in paraffin embedded mouse lung tissue using anti-NR1D1 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - analysis of NR1D1 in paraffin embedded human lung cancer tissue using anti-NR1D1 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - analysis of Rev-erb A alpha in paraffin embedded mouse lung sections using anti-Rev-erb A alpha antibody. Image from verified ...read more
Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Staining on human breast cancer tissue. Image from verified customer review.
ELISA: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Detection limit for recombinant GST tagged NR1D1 is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
4F6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Rev-erb A alpha/NR1D1 Antibody (4F6) Summary

Immunogen
NR1D1 (AAH56148, 1 a.a. ~ 615 a.a) full length recombinant protein with GST tag.MTTLDSNNNTGGVITYIGSSGSSPSRTSPESLYSDNSNGSFQSLTQGCPTYFPPSPTGSLTQDPARSFGSIPPSLSDDGSPSSSSSSSSSSSSFYNGSPPGSLQVAMEDSSRVSPSKSTSNITKLNGMVLLCKVCGDVASGFHYGVHACEGCKGFFRRSIQQNIQYKRCLKNENCSIVRINRNRCQQCRFKKCLSVGMSRDAVRFGRIPKREKQRMLAEMQSAMNLANNQLSSQCPLETSPTQHPTPGPMGPSPP
Specificity
NR1D1 (4F6)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NR1D1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Antibody reactive against recominant protein and cell lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), RNAi validation and ELISA.
Positive Control
HeLa Lysate (NB800-PC9)
Reviewed Applications
Read 4 Reviews rated 4.3
using
H00009572-M02 in the following applications:

Publications
Read Publications using H00009572-M02.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Rev-erb A alpha/NR1D1 Antibody (4F6)

  • Ear-1
  • EAR1nuclear receptor subfamily 1 group D member 1
  • HREV
  • NR1D1
  • nuclear receptor subfamily 1, group D, member 1
  • Reverb A alpha
  • Rev-erb A alpha
  • Rev-ErbAalpha
  • Rev-erbA-alpha
  • THRA1
  • THRAL
  • THRALrev-erbA-alpha
  • V-erbA-related protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00009572-M02
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for Rev-erb A alpha/NR1D1 Antibody (H00009572-M02)(2)

Reviews for Rev-erb A alpha/NR1D1 Antibody (H00009572-M02) (4) 4.34

Average Rating: 4.3
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Mouse.
We have 4 reviews tested in 2 application: IF, IHC-P.

Reviews using H00009572-M02:
Filter by Applications
IF
(1)
IHC-P
(3)
All Applications
Filter by Species
Human
(2)
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Rev-erb A alpha/NR1D1 H00009572-M02
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reviewed by:
Anonymous
IF Mouse 02/26/2015
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Summary

ApplicationImmunofluorescence
Sample TestedMouse lung paraffin embedded sections
SpeciesMouse
LotDC191-4F6

Blocking

Blocking Details10% Donkey serum for 1 hr RT

Primary Anitbody

Dilution Ratio1:250 dilution Primary antibody O/N at 4 degree C

Secondary Antibody

Secondary DescriptionDonkey-anti-mouse alexa 488
Secondary Manufacturer Cat#Donkey anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate
Secondary Concentration1:1000 dilution

Details

Detection NotesFluorescence microscope, exposure time 5000 ms
Fixation DetailsAntigen Retrieval using citrate buffer with tween 20 (0.5%) pH 6.0; 95 degree C for 45 min
Wash Description1X TBS with triton x-100 (3 times)
Immunohistochemistry-Paraffin Rev-erb A alpha/NR1D1 H00009572-M02
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reviewed by:
Anonymous
IHC-P Human 02/22/2015
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman lung cancer tissue
SpeciesHuman
LotDC191-4F6
Immunohistochemistry-Paraffin Rev-erb A alpha/NR1D1 H00009572-M02
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reviewed by:
Anonymous
IHC-P Mouse 02/22/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse lung paraffin embedded sections
SpeciesMouse
LotDC191-4F6
Immunohistochemistry-Paraffin Rev-erb A alpha/NR1D1 H00009572-M02
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reviewed by:
Hyelin Na
IHC-P Human 09/26/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman Breast Cancer Tissue
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rev-erb A alpha/NR1D1 Antibody (H00009572-M02) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4.3
5
1
4
3
3
0
2
0
1
0

Anonymous
02/26/2015
Application: IF
Species: Mouse

Anonymous
02/22/2015
Application: IHC-P
Species: Human

Anonymous
02/22/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol NR1D1
Entrez
OMIM
Uniprot