Rev-erb A alpha/NR1D1 Antibody (4F6)

Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - NR1D1 monoclonal antibody (M02), clone 4F6. Analysis of NR1D1 expression in NIH/3T3.

Immunocytochemistry/Immunofluorescence: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Analysis of monoclonal antibody to NR1D1 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Representative tissue microarray specimens stained for NR1D1. Immunoreactivity of representative tumor specimens is shown. Low and high NR1D1 expression groups are defined in methods. The low NR1D1 expression ranges from immunoreactive scores 2 to 6 (left (P2xI1), middle (P3xI1), and right (P3xI2)), and the high NR1D1 expression shows immunoreactive score 12 (all, P4xI3). Image collected and cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/s13058-019-1197-x) licensed under a CC-BY licence.

Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Analysis of NR1D1 expression in transfected 293T cell line by NR1D1 monoclonal antibody (M02), clone 4F6. Lane 1: NR1D1 transfected lysate (66.8 KDa). Lane 2: Non-transfected lysate.

Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Analysis of NR1D1 over-expressed 293 cell line, cotransfected with NR1D1 Validated Chimera RNAi ( Cat # H00009572-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NR1D1 monoclonal antibody (M02), clone 4F6 (Cat # H00009572-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - NR1D1 monoclonal antibody (M02), clone 4F6 Analysis of NR1D1 expression in Hela S3 NE.

Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - analysis of NR1D1 in paraffin embedded mouse lung tissue using anti-NR1D1 antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - analysis of NR1D1 in paraffin embedded human lung cancer tissue using anti-NR1D1 antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - analysis of Rev-erb A alpha in paraffin embedded mouse lung sections using anti-Rev-erb A alpha antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Staining on human breast cancer tissue. Image from verified customer review.

Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Analysis of monoclonal antibody to NR1D1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.

ELISA: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Detection limit for recombinant GST tagged NR1D1 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, KD
• Clone: 4F6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Rev-erb A alpha/NR1D1 Antibody (4F6) Summary

• Immunogen: NR1D1 (AAH56148, 1 a.a. ~ 614 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTTLDSNNNTGGVITYIGSSGSSPSRTSPESLYSDNSNGSFQSLTQGCPTYFPPSPTGSLTQDPARSFGSIPPSLSDDGSPSSSSSSSSSSSSFYNGSPPGSLQVAMEDSSRVSPSKSTSNITKLNGMVLLCKVCGDVASGFHYGVHACEGCKGFFRRSIQQNIQYKRCLKNENCSIVRINRNRCQQCRFKKCLSVGMSRDAVRFGRIPKREKQRMLAEMQSAMNLANNQLSSQCPLETSPTQHPTPGPMGPSPPPAPVPSPLVGFSQFPQQLTPPRSPSPEPTVEDVISQVARAHREIFTYAHDKLGSSPGNFNANHASGSPPATTPHRWENQGCPPAPNDNNTLAAQRHNEALNGLRQAPSSYPPTWPPGPAHHSCHQSNSNGHRLCPTHVYAAPEGKAPANSPRQGNSKNVLLACPMNMYPHGRSGRTVQEIWEDFSMSFTPAVREVVEFAKHIPGFRDLSQHDQVTLLKAGTFEVLMVRFASLFNVKDQTVMFLSRTTYSLQELGAMGMGDLLSAMFDFSEKLNSLALTEEELGLFTAVVLVSADRSGMENSASVEQLQETLLRALRALVLKNRPLETSRFTKLLLKLPDLRTLNNMHSEKLLSFRVDAQ
• Specificity: NR1D1 (4F6)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NR1D1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactive against recominant protein and cell lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), RNAi validation and ELISA.
• Control:

  HeLa Nuclear Cell Lysate

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Rev-erb A alpha/NR1D1 Antibody (4F6)

• Ear-1
• EAR1nuclear receptor subfamily 1 group D member 1
• HREV
• NR1D1
• nuclear receptor subfamily 1, group D, member 1
• Reverb A alpha
• Rev-erb A alpha
• Rev-ErbAalpha
• Rev-erbA-alpha
• THRA1
• THRAL
• THRALrev-erbA-alpha
• V-erbA-related protein 1

Background

NR1D1 functions as a constitutive transcriptional repressor. Possible receptor for triiodothyronine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rev-erb A alpha/NR1D1 Antibody (H00009572-M02)(6)

Publications using H00009572-M02

• Hong H, Cheung Ym, Cao X Et Al. REV-ERB alpha agonist SR9009 suppresses IL-1 beta production in macrophages through BMAL1-dependent inhibition of inflammasome Biochemical pharmacology 2021-07-26 [PMID: 34324866] (WB)
• Na H, Han J, Ka NL et al. High expression of NR1D1 is associated with good prognosis in triple-negative breast cancer patients treated with chemotherapy Breast Cancer Res. 2019-11-28 [PMID: 31779659] (IHC-P, Human)
• Bostrom J, Sramkova Z, Sala?ova A et al. Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells. PLoS One 2017-12-11 [PMID: 29228002]
• DeBruyne JP, Baggs JE, Sato TK, Hogenesch JB. Ubiquitin ligase Siah2 regulates RevErbalpha degradation and the mammalian circadian clock. Proc Natl Acad Sci U S A. 2015-09-21 [PMID: 26392558]
• Nakabayashi H, Ohta Y, Yamamoto M et al. Clock-controlled output gene Dbp is a regulator of Arnt/Hif-1b gene expression in pancreatic islet b-cells. Biochem Biophys Res Commun. 2013-04-06 [PMID: 23567972]
• Shrestha, N. The Neural Basis of a Circadian Oscillator Underlying Time Memory Thesis 2021-01-01 (IHC, Mouse)

Reviews for Rev-erb A alpha/NR1D1 Antibody (H00009572-M02) (4)

Average Rating: 4.3

(Based on 4 reviews)

We have 4 reviews tested in 2 species: Human, Mouse.

reviewed by: Isaac Sundar IF Mouse 02/26/2015

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse lung paraffin embedded sections
• Species: Mouse
• Lot: DC191-4F6

reviewed by: Isaac Sundar IHC-P Human 02/22/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human lung cancer tissue
• Species: Human
• Lot: DC191-4F6

reviewed by: Isaac Sundar IHC-P Mouse 02/22/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse lung paraffin embedded sections
• Species: Mouse
• Lot: DC191-4F6

reviewed by: Verified Customer IHC-P Human 09/26/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human Breast Cancer Tissue
• Species: Human

Bioinformatics

• Gene Symbol: NR1D1
• Entrez:
  • 9572(Human)
• OMIM:
  • 602408(Human)
• Uniprot:
  • AAH56148(Human)