EAAT2/GLT1 Antibody - BSA Free

Western Blot: EAAT2/GLT1 Antibody [NBP3-13348] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EAAT2/GLT1 antibody (NBP3-13348) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP3-13348] - EAAT2/GLT1 antibody detects EAAT2/GLT1 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse hippocampus. EAAT2/GLT1 stained by EAAT2/GLT1 antibody (NBP3-13348) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP3-13348] - EAAT2/GLT1 antibody detects EAAT2/GLT1 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: EAAT2/GLT1 stained by EAAT2/GLT1 antibody (NBP3-13348) diluted at 1:250. Red: beta Tubulin 3/ Tuj1, a cytoplasm marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Mu, Rt, Fe, RM
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

EAAT2/GLT1 Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of mouse EAAT2/GLT1. The exact sequence is proprietary.
• Predicted Species: Feline (90%), Rhesus Macaque (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC1A2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Reactivity Notes

Human (88%), Dog (88%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for EAAT2/GLT1 Antibody - BSA Free

• EAAT2
• GLT1
• member 2
• SLC1A2
• solute carrier family 1 (glial high affinity glutamate transporter), member 2
• Solute carrier family 1 member 2

Background

GLT1 is the major glial glutamate transporter that takes up more than 90% of glutamate from the synapse. GLT1b is a splice variant that contains 11 unique amino acids at the C-terminus that encode a PDZ binding domain. GLT1b was initially believed to be the neuronal isoform of GLT1, but it now appears that GLT1b is localized to astrocytes in the normal brain and to neurons in pathological conditions. In Lou Gehrig's Disease, GLT1a is decreased, while GLT1b is increased. There are also different expressions in hypoxic cells, which have not yet been fully elucidated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC1A2