E6AP/UBE3A Antibody

Images

 
Western Blot: E6AP/UBE3A Antibody [NB500-240] - UBE3A Antibody [NB500-240] - Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

E6AP/UBE3A Antibody Summary

Immunogen
A synthetic peptide mapped to a region between residue 400 and 450 of human Ubiquitin Protein Ligase E3A using the numbering given in entry NP_000453.2 (GeneID 7337).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBE3A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunoprecipitation 1-4 ug/mg of lysate
Positive Control
E6AP/UBE3A Lysate (NBL1-17551)
Publications
Read Publications using NB500-240.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for E6AP/UBE3A Antibody

  • ANCR
  • ANCREPVE6AP
  • AS
  • CTCL tumor antigen se37-2
  • E6AP ubiquitin-protein ligase
  • E6AP
  • E6-AP
  • EC 6.3.2.-
  • EPVE6AP
  • FLJ26981
  • HPVE6A
  • human papilloma virus E6-associated protein
  • Human papillomavirus E6-associated protein
  • Oncogenic protein-associated protein E6-AP
  • Renal carcinoma antigen NY-REN-54
  • UBE3A
  • ubiquitin protein ligase E3A
  • ubiquitin-protein ligase E3A

Background

E6AP was originally identified as the ubiquitin-protein ligase involved in human papillomavirus E6-mediated p53 degradation. It has since been shown to act as an E3 ubiquitin-protein ligase catalyzing the ubiquitination of several other protein substrates. Ubiquitin E3 ligases are overexpressed in many human cancers and their inhibition leads to growth suppression or apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IP

Publications for E6AP/UBE3A Antibody (NB500-240)(8)


Showing Publications 1 - 8 of 8.
Publications using NB500-240 Applications Species
Daily J, Smith AG, Weeber EJ et al. Spatial and temporal silencing of the human maternal UBE3A gene. Eur J Paediatr Neurol 2012 Nov [PMID: 22560727]
Meng L, Person RE, Beaudet AL et al. Ube3a-ATS is an atypical RNA polymerase II transcript that represses the paternal expression of Ube3a. Hum Mol Genet 2012 Jul [PMID: 22493002]
Daily JL, Nash K, Jinwal U et al. Adeno-associated virus-mediated rescue of the cognitive defects in a mouse model for Angelman syndrome. PLoS One. 2011 [PMID: 22174738]
Ramamoorthy S, Dhananjayan SC, Demayo FJ et al. Isoform-specific degradation of PR-B by E6-AP is critical for normal mammary gland development. Mol Endocrinol. 2010 Nov. [PMID: 20829392]
Sato M, Stryker MP. Genomic imprinting of experience-dependent cortical plasticity by the ubiquitin ligase gene Ube3a. Proc Natl Acad Sci U S A. 2010 Mar. [PMID: 20212164]
Reelfs O, Xu YZ, Massey A et al. Thiothymidine plus low-dose UVA kills hyperproliferative human skin cells independently of their human papilloma virus status. Mol Cancer Ther. 2007 Sep. [PMID: 17876046]
Wu MY, Tsai TF, Beaudet AL et al. Deficiency of Rbbp1/Arid4a and Rbbp1l1/Arid4b alters epigenetic modifications and suppresses an imprinting defect in the PWS/AS domain. Genes Dev. 2006 Oct. [PMID: 17043311]
Jiang YH, Sahoo T, Michaelis RC et al. A mixed epigenetic/genetic model for oligogenic inheritance of autism with a limited role for UBE3A. Am J Med Genet A 2004 Nov [PMID: 15389703]

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Product General Protocols

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol UBE3A
Entrez
Uniprot