E6AP/UBE3A Antibody - BSA Free

Western Blot: E6AP/UBE3A Antibody [NB500-240] - UBE3A Antibody [NB500-240] - Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-E6AP antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. E6AP was also immunoprecipitated by rabbit anti-E6AP antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

E6AP/UBE3A Antibody - BSA Free Summary

• Immunogen: A synthetic peptide mapped to a region between residue 400 and 450 of human Ubiquitin Protein Ligase E3A using the numbering given in entry NP_000453.2 (GeneID 7337).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBE3A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-4 ug/mg of lysate
  • Western Blot 1:1000-1:10000
• Control:

  E6AP/UBE3A Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for E6AP/UBE3A Antibody - BSA Free

• ANCR
• ANCREPVE6AP
• AS
• CTCL tumor antigen se37-2
• E6AP ubiquitin-protein ligase
• E6AP
• E6-AP
• EC 6.3.2.-
• EPVE6AP
• FLJ26981
• HPVE6A
• human papilloma virus E6-associated protein
• Human papillomavirus E6-associated protein
• Oncogenic protein-associated protein E6-AP
• Renal carcinoma antigen NY-REN-54
• UBE3A
• ubiquitin protein ligase E3A
• ubiquitin-protein ligase E3A

Background

E6AP was originally identified as the ubiquitin-protein ligase involved in human papillomavirus E6-mediated p53 degradation. It has since been shown to act as an E3 ubiquitin-protein ligase catalyzing the ubiquitination of several other protein substrates. Ubiquitin E3 ligases are overexpressed in many human cancers and their inhibition leads to growth suppression or apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for E6AP/UBE3A Antibody (NB500-240)(9)

Publications using NB500-240

• Yang S, Ting CY, Lilly MA., et Al. The GATOR2 complex maintains lysosomal-autophagic function by inhibiting the protein degradation of MiT/TFEs Mol Cell 2024-02-07 [PMID: 38325378]
• Daily J, Smith AG, Weeber EJ et al. Spatial and temporal silencing of the human maternal UBE3A gene. Eur J Paediatr Neurol 2012-11-01 [PMID: 22560727]
• Meng L, Person RE, Beaudet AL Ube3a-ATS is an atypical RNA polymerase II transcript that represses the paternal expression of Ube3a Hum Mol Genet 2012-07-01 [PMID: 22493002]
• Daily JL, Nash K, Jinwal U et al. Adeno-associated virus-mediated rescue of the cognitive defects in a mouse model for Angelman syndrome. PLoS One. 2011-01-01 [PMID: 22174738]
• Ramamoorthy S, Dhananjayan SC, Demayo FJ et al. Isoform-specific degradation of PR-B by E6-AP is critical for normal mammary gland development. Mol Endocrinol. 2010-11-01 [PMID: 20829392]
• Sato M, Stryker MP. Genomic imprinting of experience-dependent cortical plasticity by the ubiquitin ligase gene Ube3a. Proc Natl Acad Sci U S A 2010-03-01 [PMID: 20212164]
• Reelfs O, Xu YZ, Massey A et al. Thiothymidine plus low-dose UVA kills hyperproliferative human skin cells independently of their human papilloma virus status. Mol Cancer Ther. 2007-09-01 [PMID: 17876046]
• Wu MY, Tsai TF, Beaudet AL et al. Deficiency of Rbbp1/Arid4a and Rbbp1l1/Arid4b alters epigenetic modifications and suppresses an imprinting defect in the PWS/AS domain. Genes Dev. 2006-10-01 [PMID: 17043311]
• Jiang YH, Sahoo T, Michaelis RC et al. A mixed epigenetic/genetic model for oligogenic inheritance of autism with a limited role for UBE3A. Am J Med Genet A 2004-11-01 [PMID: 15389703]

Bioinformatics

• Gene Symbol: UBE3A
• Entrez:
  • 7337(Human)
  • 22215(Mouse)
• Uniprot:
  • Q05086(Human)