| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89092-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08913 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38463-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-99342-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98953-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21827-50ul | |||
![Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - UBE3A monoclonal antibody (M01), clone 2F6. Analysis of UBE3A expression in PC-12.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-2F6-Western-Blot-H00007337-M01-img0014.jpg "Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - UBE3A monoclonal antibody (M01), clone 2F6. Analysis of UBE3A expression in PC-12.")
![Immunocytochemistry/Immunofluorescence: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - Analysis of monoclonal antibody to UBE3A on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-2F6-Immunocytochemistry-Immunofluorescence-H00007337-M01-img0010.jpg)
![Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - Analysis of monoclonal antibody to UBE3A on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-2F6-Immunohistochemistry-Paraffin-H00007337-M01-img0012.jpg)
![Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - UBE3A expression in Jurkat ( Cat # L017V1 ).](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-2F6-Western-Blot-H00007337-M01-img0008.jpg)
![ELISA: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - Detection limit for recombinant GST tagged UBE3A is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-2F6-ELISA-H00007337-M01-img0015.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - Effect of E6AP silencing on the stability of HPV E6. (A)E6AP knockdown was confirmed by immunoblotting. (B) HEK293T cells (E6AP-null) were transfected with HPV16 E6 or HPV18 E6. (C) After 48 h the cells were harvested, & protein levels analyzed by immunoblotting. beta -actin acted as a control for transfection efficiency. (D) HEK293T cells (E6AP-null) were transfected with the indicated expression plasmids; after 36 h they were treated with 10 μM MG132 & 12 h later, harvested & subjected to immunoprecipitation (IP) with anti-myc-conjugated agarose beads. Polyubiquitinated HPV E6 was then detected for IP with an antibody against HA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31749782), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00007337-m01_mouse-monoclonal-e6ap-ube3a-antibody-2f6-310202415371978.jpg)
![Western Blot: E6AP/UBE3A Antibody [NB500-240] - UBE3A Antibody [NB500-240] - Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-E6AP antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. E6AP was also immunoprecipitated by rabbit anti-E6AP antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Western-Blot-NB500-240-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: E6AP/UBE3A Antibody [NB500-240] - UBE3A Antibody [NB500-240] - Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-E6AP antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. E6AP was also immunoprecipitated by rabbit anti-E6AP antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).")
![Immunocytochemistry/Immunofluorescence: E6AP/UBE3A Antibody [NBP1-89092] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89092-img0007.jpg "Immunocytochemistry/Immunofluorescence: E6AP/UBE3A Antibody [NBP1-89092] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP1-89092] - Staining of human kidney shows moderate nuclear and cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Immunohistochemistry-Paraffin-NBP1-89092-img0013.jpg)
![Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP1-89092] - Staining of human testis shows moderate nuclear and cytoplasmic postivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Immunohistochemistry-Paraffin-NBP1-89092-img0010.jpg)
![Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP1-89092] - Staining of human cerebral cortex shows moderate nuclear and cytoplasmic positivity in neuronal and glial cells.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Immunohistochemistry-Paraffin-NBP1-89092-img0011.jpg)
![Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP1-89092] - Staining of human skeletal muscle shows moderate nuclear and cytoplasmic postivity in myocytes.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Immunohistochemistry-Paraffin-NBP1-89092-img0012.jpg)
![Western Blot: E6AP/UBE3A Antibody [NBP1-89092] -Analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/antibody/nbp1-89092_rabbit-polyclonal-e6ap-ube3a-antibody-western-blot-238202383532..jpg)
![Immunocytochemistry/Immunofluorescence: E6AP/UBE3A Antibody (PCRP-UBE3A-1A2) [NBP3-07170] - Immunofluorescent Analysis of PFA-fixed HeLa cells. E6AP/UBE3A Mouse Monoclonal Antibody (PCRP-UBE3A-1A2) followed by IgG-CF488 (green), counterstained with phalloidin.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-PCRP-UBE3A-1A2-Immunocytochemistry-Immunofluorescence-NBP3-07170-img0003.jpg "Immunocytochemistry/Immunofluorescence: E6AP/UBE3A Antibody (PCRP-UBE3A-1A2) [NBP3-07170] - Immunofluorescent Analysis of PFA-fixed HeLa cells. E6AP/UBE3A Mouse Monoclonal Antibody (PCRP-UBE3A-1A2) followed by IgG-CF488 (green), counterstained with phalloidin.")
![Flow Cytometry: E6AP/UBE3A Antibody (PCRP-UBE3A-1A2) [NBP3-07170] - Flow Cytometric Analysis of PFA-fixed HeLa cells. E6AP/UBE3A Mouse Monoclonal Antibody (PCRP-UBE3A-1A2) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-PCRP-UBE3A-1A2-Flow-Cytometry-NBP3-07170-img0002.jpg)
![Protein Array: E6AP/UBE3A Antibody (PCRP-UBE3A-1A2) [NBP3-07170] - Analysis of Protein Array containing more than 19,000 full-length human proteins using E6AP/UBE3A Mouse Monoclonal Antibody (PCRP-UBE3A-1A2).](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-PCRP-UBE3A-1A2-Protein-Array-NBP3-07170-img0001.jpg)
![Western Blot: E6AP/UBE3A Antibody [NB500-239] - Detection of Human and Mouse E6AP by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-E6AP antibody NB500-239 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Western-Blot-NB500-239-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: E6AP/UBE3A Antibody [NB500-239] - Detection of Human and Mouse E6AP by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-E6AP antibody NB500-239 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: E6AP/UBE3A Antibody [NB500-239] - Cytoplasmic (S100) fraction (10 mg) from HeLa cells. Antibody used at 20 ug/10 mg extract.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Western-Blot-NB500-239-img0002.jpg)
![Western Blot: E6AP/UBE3A Antibody [NBP2-38463] - Analysis using Anti-UBE3A antibody NBP2-38463 (A) shows similar pattern to independent antibody NBP1-89092 (B).](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Western-Blot-NBP2-38463-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: E6AP/UBE3A Antibody [NBP2-38463] - Analysis using Anti-UBE3A antibody NBP2-38463 (A) shows similar pattern to independent antibody NBP1-89092 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: E6AP/UBE3A Antibody [NBP2-38463] - Staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Immunohistochemistry-NBP2-38463-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: E6AP/UBE3A Antibody [NBP2-99342] - Immunofluorescence staining of E6AP/UBE3A in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human E6AP/UBE3A polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus .](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99342-img0001.jpg "Immunocytochemistry/Immunofluorescence: E6AP/UBE3A Antibody [NBP2-99342] - Immunofluorescence staining of E6AP/UBE3A in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human E6AP/UBE3A polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus .")
![Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP2-99342] - Immunochemical staining of human E6AP/UBE3A in human testis with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Immunohistochemistry-Paraffin-NBP2-99342-img0003.jpg)
![Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP2-99342] - Immunochemical staining of human E6AP/UBE3A in human ovarian cancer with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Immunohistochemistry-Paraffin-NBP2-99342-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: E6AP/UBE3A Antibody [NBP2-98953] - Immunofluorescence staining of E6AP/UBE3A in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human E6AP/UBE3A polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98953-img0001.jpg "Immunocytochemistry/Immunofluorescence: E6AP/UBE3A Antibody [NBP2-98953] - Immunofluorescence staining of E6AP/UBE3A in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human E6AP/UBE3A polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.")
![Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP2-98953] - Immunochemical staining of human E6AP/UBE3A in human breast carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Immunohistochemistry-Paraffin-NBP2-98953-img0002.jpg)
![Western Blot: E6AP/UBE3A Antibody (SR1023) [NBP3-21827] - Western blot analysis using NBP3-21827 in K562 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21827_rabbit-e6ap-ube3a-mab-sr1023-5720231754883.jpg "Western Blot: E6AP/UBE3A Antibody (SR1023) [NBP3-21827] - Western blot analysis using NBP3-21827 in K562 cell lysate.")
![Western Blot: UBE3A Overexpression Lysate (Adult Normal) [NBL1-17551] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBE3A.](http://images.novusbio.com/fullsize/UBE3A-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17551-img0002.jpg "Western Blot: UBE3A Overexpression Lysate (Adult Normal) [NBL1-17551] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBE3A.")