E6AP/UBE3A Antibody (SR1023) - BSA Free

Western Blot: E6AP/UBE3A Antibody (SR1023) [NBP3-21827] - Western blot analysis using NBP3-21827 in K562 cell lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: SR1023
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

E6AP/UBE3A Antibody (SR1023) - BSA Free Summary

• Description: Novus Biologicals Rabbit E6AP/UBE3A Antibody (SR1023) - BSA Free (NBP3-21827) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human E6AP/UBE3A (Uniprot #: Q05086)
• Specificity: Detects endogenous levels of total E6AP/UBE3A
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: UBE3A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for E6AP/UBE3A Antibody (SR1023) - BSA Free

• ANCR
• ANCREPVE6AP
• AS
• CTCL tumor antigen se37-2
• E6AP ubiquitin-protein ligase
• E6AP
• E6-AP
• EC 6.3.2.-
• EPVE6AP
• FLJ26981
• HPVE6A
• human papilloma virus E6-associated protein
• Human papillomavirus E6-associated protein
• Oncogenic protein-associated protein E6-AP
• Renal carcinoma antigen NY-REN-54
• UBE3A
• ubiquitin protein ligase E3A
• ubiquitin-protein ligase E3A

Background

UBE3A encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBE3A