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E2F7 Antibody

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Western Blot: E2F7 Antibody [NBP2-92216] - Analysis of extracts of various cell lines, using E2F7 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Western Blot: E2F7 Antibody [NBP2-92216] - Analysis of extracts of various cell lines, using E2F7 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per ...read more
Western Blot: E2F7 Antibody [NBP2-92216] - Analysis of extracts of various cell lines, using E2F7 .Exposure time: 60s.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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E2F7 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 540-740 of human E2F7 (NP_976328.2).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
E2F7
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for E2F7 Antibody

  • E2F transcription factor 7
  • E2F-7
  • FLJ12981
  • transcription factor E2F7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow Cytometry: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of Hela cells with E2F1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Staining E2F1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Staining E2F1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
E2F8 Antibody
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H00079733-M01
Western Blot: E2F8 Antibody (3E9-2F5) [H00079733-M01] - Western Blot detection against Immunogen (73.15 KDa) .
(6 Publications)
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E2F-2 Antibody
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AF5119
Western blot shows lysates of HEK293 human embryonic kidney cell line. Gels were loaded with 30 μg of whole cell lysate (WCL), 20 μg of cytoplasmic (Cyto), and 10 μg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human E2F‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5119) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for E2F‑2 was detected at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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p53 Antibody (PAb
240) - BSA Free
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Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow (Intracellular): p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by APC-conjugated anti-mouse IgG secondary antibody F0101B.
(3 Reviews)
(33 Publications)
Species: Hu, Mu, Rt, Xp(-), Ye
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E2F-4 Antibody
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Immunoprecipitation: E2F-4 Antibody [NBP1-21374] - Detection of human E2F4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-E2F4 antibody NBP1-21374 used for IP at 6 ug per reaction. E2F4 was also immunoprecipitated by another rabbit anti-E2F4 antibody. For blotting immunoprecipitated E2F4, NBP1-21374 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Knockdown Validated: E2F-4 Antibody [NBP1-21374] - Immunoblots of indicated proteins in OVCAR-8 and PEA1 cells transfected with control luciferase (Luc) and two independent E2F-4 siRNAs. Image collected and cropped by CiteAb from the following publication (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6789141/) licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: E2F-4 Antibody [NBP1-21374] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-E2F4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
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H00005729-B01P
Western Blot: PTGDR Antibody [H00005729-B01P] - Analysis of PTGDR expression in transfected 293T cell line by PTGDR polyclonal antibody. Lane 1: PTGDR transfected lysate(39.6 KDa). Lane 2: Non-transfected lysate.
Flow Cytometry: PTGDR Antibody [H00005729-B01P] - Analysis of negative control 293 cells (Black) and PTGDR expressing 293 cells (Green) using PTGDR purified mouse polyclonal antibody.
(2 Publications)
Species: Hu
Applications: Flow, ICC/IF, WB
E2F3 Antibody
NBP1-80295
Western Blot: E2F3 Antibody [NBP1-80295] - Host: Rabbit. Target Name: E2F3. Sample Tissue: Human Lung Tumor. Antibody Dilution: 1ug/ml
Western Blot: E2F3 Antibody [NBP1-80295] - Host: Rabbit. Target Name: E2F3. Sample Tissue: Human OVCAR-3 Whole Cell. Antibody Dilution: 1ug/ml
Western Blot: E2F3 Antibody [NBP1-80295] - Host: Mouse. Target Name: E2F3. Sample Tissue: Mouse Small Intestine. Antibody Dilution: 1ug/ml
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Sandwich ELISA: E2F6 Antibody (1F7-A9) [H00001876-M02] - Detection limit for recombinant GST tagged E2F6 is approximately 0.03ng/ml as a capture antibody.
Western Blot: E2F6 Antibody (1F7-A9) [H00001876-M02] - Western Blot analysis of E2F6 expression in PC-12(Cat # L012V1 ).
Western Blot: E2F6 Antibody (1F7-A9) [H00001876-M02] - Western Blot analysis of E2F6 expression in HepG2 ( Cat # L019V1 ).
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Cyclin D2 Antibody
NBP3-13225
Western Blot: Cyclin D2 Antibody [NBP3-13225] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D2 antibody (NBP3-13225) diluted at 1:1000.
Immunohistochemistry-Paraffin: Cyclin D2 Antibody [NBP3-13225] - Cyclin D2 antibody detects Cyclin D2 protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. Cyclin D2 antibody (NBP3-13225) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/Immunofluorescence: Cyclin D2 Antibody [NBP3-13225] - Cyclin D2 antibody detects Cyclin D2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin D2 protein stained by Cyclin D2 antibody (NBP3-13225) diluted at 1:500. Blue: Hoechst 33342 staining.
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Applications: ICC/IF, IHC, IHC-P, WB
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Immunohistochemistry-Paraffin: TERT Antibody (2C4) [NB100-317] - Normal human pancreas showing moderate staining of exocrine cells and a subset of islets of Langerhans.
Immunohistochemistry-Paraffin: TERT Antibody (2C4) [NB100-317] - Human Pancreatic carcinoma showing strong staining of tumor cells.
Flow Cytometry: TERT Antibody (2C4) [NB100-317] - An intracellular stain was performed on Jurkat cells with TERT Antibody (2C4) NB100-317 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse IgM Alexa Fluor 488-conjugated secondary antibody.
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Simple Western: EDIL3/DEL1 Antibody [NBP1-28632] - Simple Western lane view shows a specific band for EDIL3/DEL1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunocytochemistry/Immunofluorescence: EDIL3/DEL1 Antibody [NBP1-28632] - HeLa cells stained NBP1-28632 (Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).
Western Blot: EDIL3/DEL1 Antibody [NBP1-28632] - Lane 1: Western blot on normal human brain, Lane 2: Western blot on normal mouse brain.
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FoxO1/FKHR
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NBP2-31376
Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Using the Alexa Fluor 488 direct conjugate An intracellular stain was performed on Daudi cells with FoxO1/FKHR (83N7F8) antibody NBP2-31376AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - An intracellular stain was performed on U2-OS cells with FoxO1/FKHR [83N7F8] Antibody NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - An intracellular stain was performed on RH-30 cells with FoxO1/FKHR Antibody [83N7F8] NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
(1 Review)
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Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
VEGF [HRP]
DVE00
(26 Reviews)
(600 Publications)
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Applications: ELISA
GAPDH Antibody
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Western Blot: GAPDH Antibody (1D4) [NB300-221] - Analysis of cell line lysates probed with mouse mAb to GAPDH (theoretical molecular weight: 36 kDa), NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.
Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1D4) [NB300-221] - Analysis of of HeLa cells stained with GAPDH antibody, dilution 1:100 (Green). DAPI staining of nuclear DNA (Blue). The GAPDHantibody produces strong cytoplasmic staining of healthy cells.
Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1D4) [NB300-221] - SH-SY5Y cells stained with GAPDH antibody NB300-221 (green). Nuclear DNA is stained with Hoechst dye (blue).
(19 Reviews)
(358 Publications)
Species: Av, Bv, Ca, Ch, ChHa, Dr, Eq, Fe, Ha, Hu, Mu, Ma-Op, Po, Pm, Rb, Rt, Sh, Ze
Applications: ChIP, ICC/IF, IHC, IHC-Fr, IP, KD, Simple Western, Single-Cell Western, WB
Serpin B3/SCCA1
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NBP2-45788
Immunohistochemistry: Serpin B3/SCCA1 Antibody (1A12) [NBP2-45788] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Serpin B3/SCCA1 Antibody (1A12) [NBP2-45788] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Serpin B3/SCCA1 Antibody (1A12) [NBP2-45788] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Western Blot: FZR1/CDH1 Antibody [NBP2-15840] - Cdh1 decreases upon Abeta treatment in neurons. Representative Western blot image of cdh1 after different time points in control conditions or with Abeta treatment. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep31158), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: FZR1/CDH1 Antibody [NBP2-15840] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FZR1 protein stained by FZR1 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 um.
Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP2-15840] - Mouse heart. FZR1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
(5 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB

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Bioinformatics

Gene Symbol E2F7