E2F-2 Antibody [Unconjugated]

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Western blot shows lysates of HEK293 human embryonic kidney cell line. Gels were loaded with 30 μg of whole cell lysate (WCL), 20 μg of cytoplasmic (Cyto), and 10 μg of nuclear extracts (Nuc). PVDF membrane was ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

E2F-2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human E2F-2
Glu308-Asn437
Accession # Q14209
Specificity
Detects human E2F-2 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
E2F2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for E2F-2 Antibody [Unconjugated]

  • E2F transcription factor 2
  • E2F2
  • E2F-2
  • E2F-2transcription factor E2F2

Background

E2F-2 (viral E2-associated factor 2) is a 50‑55 kDa member of the E2F/DP family of transcription factors. It is principally expressed by placenta, and forms a DNA‑activating E2F heterodimeric complex with DP-1 or -2. This complex, when active, promotes cell cycle progression. In quiescent cells, association with the retinoblastoma-tumor suppressor gene product termed pRB suppresses its activity. Human E2F‑2 is 437 amino acids (aa) in length and contains a CDK2 binding region (aa 65‑105), a DNA‑binding domain (aa 107‑196), a dimerization segment (aa 197‑289), a transactivation region (aa 359‑437), and a pRB binding domain (aa 410‑427). There are two potential alternate start sites at Met197 and Met342, and one splice variant that shows a two aa substitution for aa 349‑437. Over aa 308‑437, human E2F-2 is 72% aa identical to mouse E2F-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol E2F2
Uniprot