E2F8 Antibody (3E9-2F5) - Azide and BSA Free

Western Blot: E2F8 Antibody (3E9-2F5) [H00079733-M01] - Western Blot detection against Immunogen (73.15 KDa) .

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 3E9-2F5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

E2F8 Antibody (3E9-2F5) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse E2F8 Antibody (3E9-2F5) - Azide and BSA Free (H00079733-M01) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-E2F8 Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: E2F8 (AAH28244, 1 a.a. ~ 431 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAQLAAICKMQLEEQSSESRQKVKVQLARSGPCKPVAPLDPPVNAEMELTAPSLIQPLGMVPLIPSPLSSAVPLILPQAPSGPSYAIYLQPTQAHQSVTPPQGLSPTVCTTHSSKATGSKDSTDATTEKAANDTSKASASTRPGSLLPAPERQGAKSRTREPAGERGSKRASMLEDSGSKKKFKEDLKGLENVSATLFPSGYLIPLTQCSSLGAESILSGKENSSALSPNHRIYSSPIAGVIPVTSSELTAVNFPSFHVTPLKLMVSPTSVAAVPVGNSPALASSHPVPIQNPSSAIVNFTLQHLGLISPNVQLSASPGSGIVPVSPRIESVNVAPENAGTQQGRATNYDSPVPGQSQPNGQSVAVTGAQQPVPVTPKGSQLVAESFFRTPGGPTKPTSSSCMDFEGANKTSLGTLFVPQRKLEVSTEDVH
• Specificity: E2F8 - E2F transcription factor 8
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: E2F8
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot 1:500
• Application Notes: Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for E2F8 Antibody (3E9-2F5) - Azide and BSA Free

• E2F family member 8
• E2F transcription factor 8
• E2F-8
• FLJ23311
• transcription factor E2F8

Background

E2F transcription factors, such as E2F8, are essential for orchestrating expression of genes required for cell cycle progression and proliferation (Christensen et al., 2005 [PubMed 16179649]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for E2F8 Antibody (H00079733-M01)(6)

Publications using H00079733-M01

• de Bruin A, A Cornelissen PW, Kirchmaier BC et al. Genome-wide analysis reveals NRP1 as a direct HIF1alpha-E2F7 target in the regulation of motorneuron guidance in vivo. Nucleic Acids Res 2016-05-05 [PMID: 26681691] (WB)
• Cohen M, Vecsler M, Liberzon A et al. Unbiased transcriptome signature of in vivo cell proliferation reveals pro- and antiproliferative gene networks. Cell Cycle. 2013-09-15 [PMID: 23974109] (Human)
• Weijts BG, Bakker WJ, Cornelissen PW et al. E2F7 and E2F8 promote angiogenesis through transcriptional activation of VEGFA in cooperation with HIF1. EMBO J. 2012-10-03 [PMID: 22903062] (WB)
• Deng Q, Wang Q, Zong WY et al. E2F8 contributes to human hepatocellular carcinoma via regulating cell proliferation. Cancer Res 70(2):782-91. 2010-01-15 [PMID: 20068156]
• Panagiotis Zalmas L, Zhao X, Graham AL et al. DNA-damage response control of E2F7 and E2F8. EMBO Rep. 2008-03-01 [PMID: 18202719]
• Li J, Ran C, Li E et al. Synergistic Function of E2F7 and E2F8 Is Essential for Cell Survival and Embryonic Development. Dev Cell. 2008-01-15 [PMID: 18194653]

Bioinformatics

• Gene Symbol: E2F8
• Entrez:
  • 79733(Human)
• Uniprot:
  • AAH28244(Human)