Dynamin 2 Antibody

Western Blot: Dynamin 2 Antibody [NB300-617] - Western blot analysis of Dynamin II was performed using whole cell lysates and tissue lysate of SH-SY5Y (Lane 1), PC-12 (Lane 2), Neuro-2a (Lane 3), SK-N-AS (Lane 4), U- 87 MG (Lane 5) and Rat Brain (Lane 6). The blot was probed with Anti-Dynamin II Rabbit polyclonal Antibody and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) )Secondary Antibody, HRP conjugate. A band ~98 kDa corresponding to Dynamin II was observed across cell lines and tissue lysate tested. A non specific band of ~48 KDa was observed across cell lines and tissue lysate tested except for Neuro-2a cell line.

Immunocytochemistry/Immunofluorescence: Dynamin 2 Antibody [NB300-617] - Immunofluorescence analysis of Dynamin 2 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Trito(TM) X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Dynamin 2 Polyclonal Antibody in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Recombinant Secondary Antibody, Alexa Fluor(R) 488 conjugate, for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong(TM) Diamond Antifade Mountant with DAPI F-actin (Panel c: Red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing Cytoskeletal and cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background.

Dynamin 2 Antibody [NB300-617] - Immunofluorescence analysis of Dynamin II was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton(TM) X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Dynamin II Rabbit Polyclonal Antibody at 2 ug/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal(TM) Secondary Antibody, Alexa Fluor(R) 488 conjugate for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade(R) Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor(R) 555 Rhodamine Phalloidin . Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Dynamin 2 Antibody Summary

• Description: Novus Biologicals Rabbit Dynamin 2 Antibody (NB300-617) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Dynamin 2 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic Peptide: C S(760) P T P Q R R P V S S I H P P G R P P A(779)
• Specificity: Dynamin 2 - Synapse
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DNM2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot 1:1000
• Application Notes: WB: Detects an approx. 100 kDa protein representing Dynamin 2 from HeLa cell lysate.

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 15039342).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Dynamin 2 Antibody

• CMTDI1CMT2M
• CMTDIBdynamin II
• DI-CMTB
• DNM2
• DYN2
• DYN2EC 3.6.5.5
• Dynamin 2
• dynamin-2
• DYNII

Background

The dynamins are a family of 100 kDa GTPases transcribed from at least three separate genes. At least four mRNA splice variants for each dynamin have been described. Dynamins contain several conserved regions including the conserved, amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a coiled-coil region located in front of a proline-rich domain (PRD). The PRD is thought to mediate interactions between dynamin and numerous other cellular proteins. Dynamin 1 is expressed exclusively in neurons, Dynamin 2 is ubiquitously expressed, and Dynamin 3 is thought to be restricted to expression in the brain, testis, heart, and lung. The dynamins participate in the cellular process of clathrin-mediated and fluid-phase endocytosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dynamin 2 Antibody (NB300-617)(3)

Publications using NB300-617

• Chou Ai Mei, Sem Kai Ping, Wright Graham Daniel et al. Dynamin1 is a novel target for IRSp53 protein and works with mammalian enabled (Mena) protein and Eps8 to regulate filopodial dynamics. J Biol Chem. 2014-08-29 [PMID: 25031323] (Human)
• Jeon J, Leibiger I, Moede T et al. Dynamin-mediated Nephrin Phosphorylation Regulates Glucose-stimulated Insulin Release in Pancreatic Beta Cells J Biol Chem 2012-08-17 [PMID: 22718751] (WB, Human)
• Min L, Leung YM, Tomas A et al. Dynamin Is Functionally Coupled to Insulin Granule Exocytosis. J Biol Chem 2007-11-16 [PMID: 17848579]

Bioinformatics

• Gene Symbol: DNM2
• Entrez:
  • 1785(Human)
  • 25751(Rat)