Dynamin 2 Antibody (1D8) - BSA Free

Western Blot: Dynamin 2 Antibody (1D8) [NBP3-26615] - Positive Western Blot detected in: HL60 whole cell lysate, Rat brain tissue. All lanes: Dynamin 2 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 99, 98 kDa Observed band size: 99 kDa

Immunocytochemistry/Immunofluorescence: Dynamin 2 Antibody (1D8) [NBP3-26615] - Staining of Hela Cells with Dynamin 2 Antibody (1D8) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Flow Cytometry: Dynamin 2 Antibody (1D8) [NBP3-26615] - Overlay histogram showing Hela cells stained with Dynamin 2 Antibody (1D8) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 1D8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Dynamin 2 Antibody (1D8) - BSA Free Summary

• Description: Novus Biologicals Rabbit Dynamin 2 Antibody (1D8) - BSA Free (NBP3-26615) is a recombinant monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human Dynamin 2 [UniProt P50570]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DNM2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:20-1:200
  • Immunocytochemistry/ Immunofluorescence 1:20-1:200
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Dynamin 2 Antibody (1D8) - BSA Free

• CMTDI1CMT2M
• CMTDIBdynamin II
• DI-CMTB
• DNM2
• DYN2
• DYN2EC 3.6.5.5
• Dynamin 2
• dynamin-2
• DYNII

Background

The dynamins are a family of 100 kDa GTPases transcribed from at least three separate genes. At least four mRNA splice variants for each dynamin have been described. Dynamins contain several conserved regions including the conserved, amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a coiled-coil region located in front of a proline-rich domain (PRD). The PRD is thought to mediate interactions between dynamin and numerous other cellular proteins. Dynamin 1 is expressed exclusively in neurons, Dynamin 2 is ubiquitously expressed, and Dynamin 3 is thought to be restricted to expression in the brain, testis, heart, and lung. The dynamins participate in the cellular process of clathrin-mediated and fluid-phase endocytosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DNM2