Dynactin Subunit 2/DCTN2/DCTN-50 Antibody

Western Blot: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP2-16115] - Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE diluted at 1:3000

Immunocytochemistry/Immunofluorescence: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP2-16115] - Analysis of methanol-fixed MCF-7, using Dynactin 2 antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP2-16115] - Human breast cancer, using Dynactin 2 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Dynactin Subunit 2/DCTN2/DCTN-50 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Dynactin Subunit 2/DCTN2/DCTN-50. The exact sequence is proprietary.
• Localization: Cytoplasm; cytoskeleton; Membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DCTN2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody

• DCTN2
• DCTN-50
• DCTN5050 kD dynein-associated polypeptide
• dynactin 2 (p50)
• dynactin complex 50 kD subunit
• Dynactin complex 50 kDa subunit
• Dynactin Subunit 2
• dynactin subunit 2,50 kDa dynein-associated polypeptide
• Dynamitin
• p50 dynamitin
• RBP50

Background

This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (NBP2-16115)(1)

Publication using NBP2-16115

• Ayyadevara S, Balasubramaniam M, Parcon PA et al. Proteins that mediate protein aggregation and cytotoxicity distinguish Alzheimer's hippocampus from normal controls Aging Cell 2016-07-23 [PMID: 27448508]

Bioinformatics

• Gene Symbol: DCTN2
• Uniprot:
  • Q13561(Human)