DUSP23 Antibody - Azide and BSA Free

Immunochemical staining of DUSP23 in human kidney formalin-fixed paraffin embedded sections with NBP3-21438 at 1:100 dilution

Immunocytochemistry/ Immunofluorescence: DUSP23 Antibody [NBP3-21438] - Immunofluorescence staining of DUSP23 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-21438 (dilution ratio 1:100) at 4C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Nucleus and Cytoplasm.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

DUSP23 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human DUSP23 fragment
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DUSP23
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for DUSP23 Antibody - Azide and BSA Free

• dual specificity phosphatase 23
• dual specificity protein phosphatase 23
• DUSP25
• EC 3.1.3.16
• EC 3.1.3.48
• FLJ20442
• LDP3
• LDP-3
• Low molecular mass dual specificity phosphatase 3
• low-molecular-mass dual-specificity phosphatase 3
• MOSP
• RP11-190A12.1
• VH1-like member Z
• VH1-like phosphatase Z
• VHZ

Background

DUSP23 is a dual substrate specificity phosphatase that mediates dephosphorylation of proteins phosphorylated on Tyr and Ser/Thr residues. It is widely expressed and highly conserved. Most of dual-specificity protein phosphatases (DSPs) play an important role in the regulation of mitogenic signal transduction and controlling the cell cycle in response to extracellular stimuli. Reverse transcription-PCR (RT-PCR) revealed that the DUSP23 was expressed in most fetal tissues and two adult tissues: testis and colon.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DUSP23