VHR/DUSP3 Antibody (237020)

Western blot shows lysates of TE-85 human osteogenic sarcoma cell line, TF-1 human erythroleukemic cell line, and C2C12 mouse myoblast cell line . PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/ Rat VHR Monoclonal Antibody (Catalog # MAB1654) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human VHR (Catalog # 1654-VH) (2 ng) was included. A specific band for VHR was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 237020
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

VHR/DUSP3 Antibody (237020) Summary

• Immunogen: E. coli-derived recombinant human VHR
  Ser2-Pro185
  Accession # P51452
• Specificity: Detects human, mouse, and rat VHR in Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: DUSP3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VHR/DUSP3 Antibody (237020)

• dual specificity phosphatase 3
• dual specificity protein phosphatase 3
• Dual specificity protein phosphatase VHR
• DUSP3
• EC 3.1.3.16
• EC 3.1.3.48
• Vaccinia H1-related phosphatase
• vaccinia virus phosphatase VH1-related
• VHR
• VHRserine/threonine specific protein phosphatase

Background

Vaccinia Virus VH1-related Phosphatase (VHR), also known as Dual-Specificity Phosphatase 3 (DUSP3), removes inorganic phosphate groups covalently attached to tyrosine, serine and threonine residues in proteins (1). It belongs to a family of phosphatases that selectively dephosphorylate MAP kinases. VHR has been shown to dephosphorylate cellular ERK1, ERK2 and JNK, but not p38 (2, 3). Phosphorylation of VHR at tyrosine 138 by ZAP-70 enhances inhibition of ERK2, suggesting a role for VHR in modulating T-cell receptor responses (3). The enzymatic mechanism for VHR has been studied in detail (4) and its X-ray crystal structure has been characterized (5). Its well-defined biochemistry has made it useful in screening assays for compounds that inhibit phosphatases (6).

1. Ishibashi, T. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12170.
2. Todd, J.L. et al. (1999) J. Biol. Chem. 274:13271.
3. Alonso, A. et al. (2003) Nat. Immunol. 4:44.
4. Zhang, Z.-Y. et al. (1995) Biochemistry 34:16088.
5. Yuvaniyama, J. et al. (1996) Science 272:1328.
6. Ueda, K. et al. (2002) FEBS Lett. 525:48.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DUSP3
• Uniprot:
  • P51452()